| SIGNR1 ligation on murine peritoneal macrophages induces IL-12 production through NFkappaB activation. | |
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MedLine Citation:
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PMID: 20589530 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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We have previously shown that murine resident peritoneal macrophages (PEMs) are activated in response to uptake of oligomannose-coated liposomes (OMLs), leading to production of interleukin (IL)-12. To understand the mechanism of activation of PEMs by OMLs, in the present study we investigated the role of a mannose-binding C-type lectin receptor, SIGNR1, in production of proinflammatory cytokines by PEMs, in which SIGNR1 acts as a physiological receptor for OMLs. Engagement of SIGNR1 on PEMs with an anti-SIGNR1-specific rat IgM antibody, ERTR9, induced production of IL-12 and tumor necrosis factor (TNF)-alpha from PEMs, while secretion of IL-6 and IL-1beta was not detected with the same treatment. The level of phosphorylated IkappaB kinase in PEMs also increased in response to ERTR9 treatment of the cells. Treatment of PEMs with a specific nuclear factor kappa-B (NFkappaB) inhibitor, BAY11-7082, reduced ERTR9-dependent IL-12 production. Intraperitoneal treatment with BAY11-7082 also led to reduction of subsequent OML-induced IL-12 production from PEMs. These results indicate that SIGNR1-mediated intercellular signaling may induce production of cytokines such as IL-12 through NFkappaB activation. |
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Authors:
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Chiaki Kato; Naoya Kojima |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-30 |
Journal Detail:
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Title: Glycoconjugate journal Volume: 27 ISSN: 1573-4986 ISO Abbreviation: Glycoconj. J. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8603310 Medline TA: Glycoconj J Country: United States |
Other Details:
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Languages: eng Pagination: 525-31 Citation Subset: IM |
Affiliation:
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Institute of Glycoscience, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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