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SIGNR1 ligation on murine peritoneal macrophages induces IL-12 production through NFkappaB activation.
MedLine Citation:
PMID:  20589530     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
We have previously shown that murine resident peritoneal macrophages (PEMs) are activated in response to uptake of oligomannose-coated liposomes (OMLs), leading to production of interleukin (IL)-12. To understand the mechanism of activation of PEMs by OMLs, in the present study we investigated the role of a mannose-binding C-type lectin receptor, SIGNR1, in production of proinflammatory cytokines by PEMs, in which SIGNR1 acts as a physiological receptor for OMLs. Engagement of SIGNR1 on PEMs with an anti-SIGNR1-specific rat IgM antibody, ERTR9, induced production of IL-12 and tumor necrosis factor (TNF)-alpha from PEMs, while secretion of IL-6 and IL-1beta was not detected with the same treatment. The level of phosphorylated IkappaB kinase in PEMs also increased in response to ERTR9 treatment of the cells. Treatment of PEMs with a specific nuclear factor kappa-B (NFkappaB) inhibitor, BAY11-7082, reduced ERTR9-dependent IL-12 production. Intraperitoneal treatment with BAY11-7082 also led to reduction of subsequent OML-induced IL-12 production from PEMs. These results indicate that SIGNR1-mediated intercellular signaling may induce production of cytokines such as IL-12 through NFkappaB activation.
Authors:
Chiaki Kato; Naoya Kojima
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-30
Journal Detail:
Title:  Glycoconjugate journal     Volume:  27     ISSN:  1573-4986     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  525-31     Citation Subset:  IM    
Affiliation:
Institute of Glycoscience, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan.
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