| SIAH1 inactivation correlates with tumor progression in hepatocellular carcinomas. | |
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MedLine Citation:
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PMID: 12557228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accumulation of loss of heterozygosity (LOH) on chromosome 16 is frequently observed in human hepatocellular carcinomas (HCCs). To identify tumor-suppressor genes (TSGs) involved in hepatocarcinogenesis, we performed deletion mapping of chromosome 16 in 59 HCCs. Three commonly deleted regions, located in 16q12.1, 16q22.1, and 16q24.2, were observed. Because there has been no study on LOH at locus 16q12.1 in HCCs, we focused on this region. By searching the Human Genome Database at the National Center for Biotechnology Information web site, we identified 14 known genes in 16q12.1 as TSG candidates. Among these, the expression of SIAH1 was markedly downregulated in HCCs, and inactivation of SIAH1 expression was associated with LOH at 16q12.1. A mutation analysis of SIAH1 revealed no somatic mutations, but one single nucleotide polymorphism was found among the 35 HCCs investigated. Subsequently, we evaluated the relation between SIAH1 expression, confirmed by semiquantitative RT-PCR, and clinicopathological parameters in HCCs. SIAH1 was significantly downregulated in advanced HCCs, including poorly differentiated tumors, larger tumors, and tumors in advanced stages. These findings suggest that inactivation of SIAH1 plays an important role in HCC progression. |
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Authors:
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Koichi Matsuo; Seiji Satoh; Hiroshi Okabe; Akinari Nomura; Toshiki Maeda; Yoshio Yamaoka; Iwao Ikai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes, chromosomes & cancer Volume: 36 ISSN: 1045-2257 ISO Abbreviation: Genes Chromosomes Cancer Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-01-30 Completed Date: 2003-03-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9007329 Medline TA: Genes Chromosomes Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 283-91 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Wiley-Liss, Inc. |
Affiliation:
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Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan. kmatsuo@kuhp.kyoto-u.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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genetics*,
pathology Chromosome Deletion Chromosome Mapping Chromosomes, Human, Pair 16 / genetics DNA Mutational Analysis Databases, Genetic Disease Progression Female Gene Expression Profiling Gene Silencing* Humans Liver Neoplasms / genetics*, pathology Loss of Heterozygosity / genetics Male Middle Aged Nuclear Proteins / antagonists & inhibitors*, genetics* Sequence Homology, Nucleic Acid Tumor Cells, Cultured Ubiquitin-Protein Ligases |
| Chemical | |
Reg. No./Substance:
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0/Nuclear Proteins; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/seven in absentia proteins |
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