| SFTG international collaborative study on in vitro micronucleus test I. General conditions and overall conclusions of the study. | |
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MedLine Citation:
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PMID: 16815079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study, coordinated by the SFTG (French branch of European Environmental Mutagen Society), included 38 participants from Europe, Japan and America. Clastogens (bleomycin, urethane), including base and nucleoside analogs (5-fluorouracil and cytosine arabinoside), aneugens and/or polyploidy inducers (colchicine, diethylstilboestrol, griseofulvin and thiabendazole), as well as non-genotoxic compounds (mannitol and clofibrate), were tested. Four cell types were used, i.e. human lymphocytes in the presence of cytochalasin B and CHO, CHL and L5178Y cell lines, in the presence or absence of cytochalasin B, with various treatment-recovery schedules. Mitomycin C was used as a positive control for all cell types. Mannitol and clofibrate were consistently negative in all cell types and with all treatment-recovery conditions. Urethane, known to induce questionable clastogenicity, was not found as positive. Bleomycin and mitomycin C were found positive in all treatment-recovery conditions. The base and nucleoside analogs were less easy to detect, especially 5-fluorouracil due to the interference with cytotoxicity, while cytosine arabinoside was detected in all cell types depending on the treatment-recovery schedule. Aneugens (colchicine, diethylstilboestrol and griseofulvin) were all detected in all cell types. In this study, the optimal detection was ensured when a short treatment followed by a long recovery was associated with a long continuous treatment without recovery. There was no impact of the presence or absence of cytochalasin B on the detection of micronucleated cells on cell lines. Scoring micronucleated cells in both mononucleated and binucleated cells when using cytochalasin B was confirmed to be useful for the detection and the identification of aneugens. In conclusion, these results, together with previously published validation studies, provide a useful contribution to the optimisation of a study protocol for the detection of both clastogens and aneugens in the in vitro micronucleus test. |
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Authors:
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Elisabeth Lorge; Veronique Thybaud; Marilyn J Aardema; Jo Oliver; Akihiro Wakata; Giocondo Lorenzon; Daniel Marzin |
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Publication Detail:
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Type: Evaluation Studies; In Vitro; Journal Article; Validation Studies Date: 2006-06-30 |
Journal Detail:
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Title: Mutation research Volume: 607 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-17 Completed Date: 2006-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 13-36 Citation Subset: IM |
Affiliation:
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Servier Group, Drug Safety Assessment, Orleans-Gidy, France. elisabeth.lorge@fr.netgrs.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aneugens
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toxicity Animals Bleomycin / toxicity CHO Cells Cell Line Clofibrate / toxicity Colchicine / toxicity Cricetinae Cytarabine / toxicity Cytochalasin B Diethylstilbestrol / toxicity Fluorouracil / toxicity Griseofulvin / toxicity Humans International Cooperation Leukemia L5178 Lymphocytes / drug effects Mannitol / toxicity Mice Micronucleus Tests / methods*, standards Mitomycin / toxicity Mutagens / toxicity Thiabendazole / toxicity |
| Chemical | |
Reg. No./Substance:
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0/Aneugens; 0/Mutagens; 11056-06-7/Bleomycin; 126-07-8/Griseofulvin; 147-94-4/Cytarabine; 148-79-8/Thiabendazole; 14930-96-2/Cytochalasin B; 50-07-7/Mitomycin; 51-21-8/Fluorouracil; 56-53-1/Diethylstilbestrol; 637-07-0/Clofibrate; 64-86-8/Colchicine; 69-65-8/Mannitol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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