Document Detail


Comparative analysis ofCas6b processing and CRISPR RNA stability.
MedLine Citation:
PMID:  23392318     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prokaryotic antiviral defense systems CRISP R (clustered regularly interspaced short palindromic repeats)/Cas (CRISP Rassociated) employs short crRNAs (CRISP R RNAs) to target invading viral nucleic acids. A short spacer sequence of these crRNAs can be derived from a viral genome and recognizes a reoccurring attack of a virus via base complementarity. We analyzed the effect of spacer sequences on the maturation of crRNAs of the subtype I-B Methanococcus maripaludis C5 CRISP R cluster. The responsible endonuclease, termed Cas6b, bound non-hydrolyzable repeat RNA as a dimer and mature crRNA as a monomer. Comparative analysis of Cas6b processing of individual spacer-repeat-spacer RNA substrates and crRNA stability revealed the potential influence of spacer sequence and length on these parameters. Correlation of these observations with the variable abundance of crRNAs visualized by deep-sequencing analyses is discussed. Finally, insertion of spacer and repeat sequences with archaeal poly-T termination signals is suggested to be prevented in archaeal CRISP R/Cas systems.
Authors:
Hagen Richter; Sita J Lange; Rolf Backofen; Lennart Randau
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  RNA biology     Volume:  10     ISSN:  1555-8584     ISO Abbreviation:  RNA Biol     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-09-13     Completed Date:  2014-01-28     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101235328     Medline TA:  RNA Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  700-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry*,  genetics,  metabolism
Base Sequence
CRISPR-Associated Proteins / chemistry*,  metabolism*
Clustered Regularly Interspaced Short Palindromic Repeats* / genetics
Methanococcus / genetics,  metabolism*
Molecular Sequence Data
Protein Multimerization
RNA Stability*
RNA, Bacterial / genetics*,  metabolism*
RNA-Binding Proteins / chemistry,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/CRISPR-Associated Proteins; 0/RNA, Bacterial; 0/RNA-Binding Proteins
Comments/Corrections

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