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SF-1 is required for functional maturation of Sertoli cells during postnatal development.
MedLine Citation:
PMID:  22419830     Owner:  NLM     Status:  Publisher    
The orphan nuclear receptor steroidogenic factor 1 (SF-1) is expressed in both Sertoli and Leydig cells in testes. The present study investigates postnatal development of the testes of a gonad-specific Sf-1 knockout (KO) mouse, in which Sf-1 was specifically inactivated. The KO testes appeared histologically normal from postnatal day (P) 0 until P7. However, disorganized germ cells, vacuoles, and giant cells appeared by P14 in the seminiferous tubules of KO, but not control mice. Expression of SF-1 and various factors was examined by immunohistochemistry (IHC). The number of SF-1-positive Sertoli cells in the KO testes was lower compared to controls at all the developmental stages, and decreased to nearly undetectable levels by P21. IHC for AMH and p27, immature and mature Sertoli cell markers respectively, indicated a delay of Sertoli cell maturation in the KO testes. The number of Sertoli cells expressing factors involved in Sertoli cell differentiation including WT1, SOX9, GATA4, and androgen receptor, were lower in the KO testes compared to controls. Furthermore, fewer PCNA-positive proliferative germ cells were observed, and the number of TUNEL-labeled cells was significantly higher in the KO testes compared to controls at P14 and P21, indicating impaired spermatogenesis. IHC for SCC indicated a presence of steroidogenic Leydig cells in the interstitium of the KO testes at all stages examined. These results suggest that SF-1 is essential for Sertoli cell maturation and therefore spermatogenesis, during postnatal testis development.
Tomoko Kato; Michiyo Esaki; Ayami Matsuzawa; Yayoi Ikeda
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-14
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
T Kato, Histology and Cell Biology, Yokohama City University School of Medicine, Yokohama, Japan.
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