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SERT and TPH-1 mRNA expression are reduced in Irritable Bowel Syndrome patients irrespective of visceral sensitivity state in large intestine.
MedLine Citation:
PMID:  22323131     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Colorectal visceral hypersensitivity has been demonstrated in a subset of Irritable Bowel Syndrome (IBS) patients. Serine protease and serotonergic signalling modulate gastrointestinal visceral sensitivity. We evaluated whether altered mucosal serine protease and serotonergic pathway components are related to rectal visceral hypersensitivity in IBS patients. Colorectal mucosal biopsies of 23 IBS patients and 15 controls were collected. Gene transcripts of PAR-2, trypsinogen IV, TPH-1 and SERT were quantified using real-time PCR. Substance P and 5-HT contents were measured by ELISA. The number of enterochromaffin cells, mast cells and intraepithelial lymphocytes was determined using immunohistochemistry. Rectal visceral sensitivity was determined in IBS patients using barostat programmed for phasic ascending distension. Rectal hypersensitivity (+) and (-) IBS patients showed lower TPH-1 and SERT mRNA levels in the rectum compared to controls (P≤0.05). Rectal hypersensitivity (+) IBS patients (n=12) showed lower TPH-1 mRNA level in sigmoid compared to controls (P=0.015). No significant differences were observed in PAR-2 and trypsinogen IV expression between controls and IBS patients. Rectal substance P content was increased in IBS patients compared to controls (P=0.045). No significant differences were found in transcript levels, cell counts and substance P and 5-HT contents between rectal hypersensitivity (+) and (-) IBS patients. In conclusion, irrespective of visceral hypersensitivity state, several serotonergic signalling components are altered in IBS patients.
Authors:
Angele Pm Kerckhoffs; Jose Jm Ter Linde; Louis M A Akkermans; Melvin Samsom
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-9
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  -     ISSN:  1522-1547     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1University Medical Centre Utrecht.
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