| SEM-EDX study of prepared human dentine surfaces exposed to gingival retraction fluids. | |
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MedLine Citation:
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PMID: 16199281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To evaluate the effects of gingival retraction fluids (GRF) on prepared dentine surfaces, and to test the null-hypothesis that prior exposure of dentine surfaces for prolonged period to any of the fluids evaluated does not influence acid-etching of the exposed surfaces. METHODS: The investigation was carried out using SEM and energy-dispersive X-ray analysis (EDX). The GRF studied were Hemodent (HMDT), Astringedent (AST) and Ultradent buffered 25% Aluminium Chloride (ULTB). Longitudinal sections of 220-grit ground dentine surfaces were exposed to GRF for 0.5-, 1-, 2- and 5-min (n=4 each). Another group of samples was produced by 20s application of 35% phosphoric acid (PA) on GRF-pretreated dentine. Control samples were not exposed to GRF. Differences in etching effect-a function of the Ca-contents detected by EDX, were analysed using Friedman's and Wilcoxon's rank test (P<0.05). RESULTS: The SEM demonstrated the presence of a relatively non-porous amorphous dentine matrix, but many of the dentine tubule orifices remained occluded. Granular precipitates, which remained even after acid-etching, were noted on surfaces exposed to Hemodent for 5 min. Characteristic crystal growth was observed on surfaces exposed to Astringedent for 1- or 2-min prior to acid-etching. The EDX data demonstrated that there were differences in resulting Ca-content; ULTB >AST>HMDT>ULTB+PA>HMDT+PA>AST+PA>PA, but AST and HMDT were not significantly different. CONCLUSIONS: The exposure of dentine prepared surfaces to these three GRF altered its morphology and reduced the dentine's susceptibility to acid-etching, thus the null-hypothesis is rejected. |
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Authors:
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Olalekan A Ayo-Yusuf; Cornel H Driessen; Andre J Botha |
Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article Date: 2005-04-07 |
Journal Detail:
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Title: Journal of dentistry Volume: 33 ISSN: 0300-5712 ISO Abbreviation: J Dent Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-03 Completed Date: 2005-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0354422 Medline TA: J Dent Country: England |
Other Details:
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Languages: eng Pagination: 731-9 Citation Subset: D; IM |
Affiliation:
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Department of Community Dentistry, School of Dentistry, Room 6.43-2, Oral and Dental Hospital, University of Pretoria, P.O. box 1266, Pretoria 0001, South Africa. lekan.ayoyusuf@up.ac.za |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Etching, Dental* Dental Materials / pharmacology* Dentin / drug effects*, ultrastructure Electron Probe Microanalysis / methods Humans Microscopy, Electron, Scanning Organic Chemicals / pharmacology Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/Dental Materials; 0/Organic Chemicals; 0/Ultradent drying agent |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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