Document Detail


SELDI-TOF MS analysis of the Cardiac Troponin I forms present in plasma from patients with myocardial infarction.
MedLine Citation:
PMID:  17072908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The troponin (Tn) complex is composed of troponin T, troponin C and troponin I. The cardiac isoform of TnI (cTnI) is modified and released in blood of patients with cardiovascular diseases as a heterogeneous mixture of free, complexed and posttranslationally modified forms. With the aim to determine later, whether specific forms of cTnI could be associated with the different pathologies leading to cTnI release, the cTnI forms present in the plasma from 64 patients with acute myocardial infarction (AMI) have been analysed by SELDI-TOF MS using anti-TnI mAbs coupled to PS20 ProteinChips arrays. Upfront immunoaffinity enrichment using anti-cTnI 19C7 mAb allowed us to detect cTnI and bis-phosphorylated cTnI in 11/12 and 9/12 analyses respectively, as well as truncated cTnI in plasma with concentration of cTnI as low as 8 ng/mL. Cardiac troponin C (cTnC) and covalent TnIC complex were also found in pools of plasma with higher concentrations of cTnI. MAb 19C7-affinity SELDI-TOF MS analysis performed after immunopurification of one pool of AMI plasma with anti-free cTnI, anti-cTnC, and anti-phosphorylated cTnI mAbs indicated that intact and bis-phosphorylated cTnI were mostly under the free form. Besides, a 18 718 m/z peak could correspond to a truncated phosphorylated form initially complexed with cTnC.
Authors:
Estelle Peronnet; Laurence Becquart; Florence Poirier; Myriam Cubizolles; Geneviève Choquet-Kastylevsky; Colette Jolivet-Reynaud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proteomics     Volume:  6     ISSN:  1615-9853     ISO Abbreviation:  Proteomics     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  6288-99     Citation Subset:  IM    
Affiliation:
Unité Mixte de Recherche UMR 2714 CNRS-bioMérieux, IFR 128 BioSciences Lyon-Gerland, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Humans
Myocardial Infarction / blood*
Phosphorylation
Protein Array Analysis
Protein Isoforms / analysis*,  immunology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Troponin I / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Troponin I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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