| SCO3900, co-transcripted with three downstream genes, is involved in the differentiation of Streptomyces coelicolor. | |
| | |
MedLine Citation:
|
PMID: 20012957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
SCO3900, a putative target gene of the developmental regulator WhiI of Streptomyces coelicolor, encodes a protein similar to PadR from Pediococcus pentosaceus. The SCO3900 disruption mutant exhibited a bald phenotype with sparse aerial hyphae. However, a single copy of SCO3900 under the control of its native promoter could not complement the mutant phenotype. A fragment containing SCO3900 and its downstream three genes completely restored the mutant phenotype to the wild-type. These suggested that the disruption of SCO3900 exerted a polar effect on its downstream genes. The co-transcription of SCO3899, SCO3898, and SCO3897 with SCO3900 was confirmed by the RT-PCR. Moreover, overexpression of SCO3900 in the wild-type strain caused the same phenotype as that of the disruption mutant. The results suggested that SCO3900 encodes a negative regulator participating in morphological differentiation of S. coelicolor by influencing the expression of its downstream genes. |
| | |
Authors:
|
Guohua Zhang; Yuqing Tian; Kun Hu; Chi Feng; Huarong Tan |
Related Documents
:
|
3409557 - Anemia in a line of transgenic mice carrying a mutant dihydrofolate reductase gene. 19435937 - The ngatha genes direct style development in the arabidopsis gynoecium. 21429757 - Association of hemicellulose- and pectin-modifying gene expression with eucalyptus glob... 17938217 - Cytotoxicity in macrophages infected with rough brucella mutants is type iv secretion s... 20470417 - Large scale physiological readjustment during growth enables rapid, comprehensive and i... 18164077 - The interleukin 23 receptor gene in multiple sclerosis: a case-control study. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-15 |
Journal Detail:
|
Title: Current microbiology Volume: 60 ISSN: 1432-0991 ISO Abbreviation: Curr. Microbiol. Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-03-18 Completed Date: 2010-06-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 268-73 Citation Subset: IM |
Affiliation:
|
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Proteins
/
biosynthesis,
genetics,
physiology* Gene Deletion Gene Expression Profiling Gene Expression Regulation, Bacterial* Genetic Complementation Test Mutagenesis, Insertional Operon Reverse Transcriptase Polymerase Chain Reaction Streptomyces coelicolor / growth & development, physiology* Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Paclitaxel combined with ifosfamide in anthracycline- and docetaxel-pretreated metastatic breast can...
Next Document: Rare localizations of bone sarcoidosis: two case reports and review of the literature.