Document Detail


S1P(5) is required for sphingosine 1-phosphate-induced autophagy in human prostate cancer PC-3 cells.
MedLine Citation:
PMID:  19474291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sphingosine 1-phosphate (S1P) is a platelet- and endothelial cell-released lysophospholipid that regulates various cellular functions through activating a specific family of G protein-coupled receptors. Both platelet activation and angiogenesis play important roles in cancer development, implying that cancer cells might encounter a large amount of S1P during these processes. Cancer cells, in the meantime, may experience nutrient deprivation and rely on autophagy for early development. Whether extracellular S1P regulates autophagy remains to be tested. In the present work, we investigated whether autophagy is regulated by S1P in PC-3 cells. Through monitoring the modification patterns of LC3 by Western blotting, we demonstrated that autophagy was induced by exogenously applied S1P in PC-3 cells. This observation was further confirmed by fluorescence microscopy using PC-3 cells stably expressing enhanced green fluorescent protein-LC3. By applying small interfering RNA and dihydro-S1P, S1P(5) activation was found to be involved in this process. Besides, mammalian target of rapamycin signaling was inhibited upon S1P treatment. Taken together, our results suggest that, under serum-starved conditions, S1P further upregulates autophagic activity through S1P(5)-dependent pathways in PC-3 cells.
Authors:
Chi-Lun Chang; Ming-Chih Ho; Po-Huang Lee; Chi-Yen Hsu; Wei-Pang Huang; Hsinyu Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-27
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  297     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-09-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C451-8     Citation Subset:  IM    
Affiliation:
Institute of Zoology, National Taiwan University, Taipei, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy / physiology*
Cell Line, Tumor
Humans
Lysophospholipids / metabolism*
Male
Microtubule-Associated Proteins
Phagosomes / metabolism
Prostatic Neoplasms
Protein Kinases / metabolism
RNA, Small Interfering / genetics,  metabolism
Receptors, Lysosphingolipid / genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Ribosomal Protein S6 Kinases, 70-kDa / metabolism
Signal Transduction / physiology
Sphingosine / analogs & derivatives*,  metabolism
Chemical
Reg. No./Substance:
0/Lysophospholipids; 0/Microtubule-Associated Proteins; 0/RNA, Small Interfering; 0/Receptors, Lysosphingolipid; 0/Recombinant Fusion Proteins; 0/light chain 3, human; 123-78-4/Sphingosine; 26993-30-6/sphingosine 1-phosphate; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein; EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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