Document Detail


S100beta and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury.
MedLine Citation:
PMID:  19030756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100beta are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100beta are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100beta positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100beta positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100beta may be important in wound repair and restorative events in the lesioned peripheral nerves.
Authors:
Tatiana Duobles; Thais de Sousa Lima; Beatriz de Freitas Azevedo Levy; Gerson Chadi
Related Documents :
16632316 - Olfactory ensheathing cells: ripples of an incoming tide?
7722636 - Temporal relations of the complex spike activity of purkinje cell pairs in the vestibul...
8116476 - Requirements for schwann cell migration within cns environments: a viewpoint.
12504876 - Development of a corticotropin-releasing factor-mediated effect on the firing rate of p...
9676576 - Cell biology of astrocyte proteoglycans.
12846556 - Differentiation and proliferation of endocrine cells in the regenerating rat pancreas a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia     Volume:  23     ISSN:  1678-2674     ISO Abbreviation:  Acta Cir Bras     Publication Date:    2008 Nov-Dec
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9103983     Medline TA:  Acta Cir Bras     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  555-60     Citation Subset:  IM    
Affiliation:
Department of Neurology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Axotomy
Blotting, Western
Cells, Cultured
Fibroblast Growth Factor 2 / analysis,  metabolism*
Ganglia, Spinal / chemistry,  cytology,  metabolism*
Immunohistochemistry
Male
Nerve Crush
Nerve Growth Factors / analysis,  metabolism*
Paracrine Communication
Peripheral Nerves / injuries*,  physiology,  surgery
Rats
Rats, Wistar
S100 Proteins / analysis,  metabolism*
Satellite Cells, Perineuronal / metabolism
Schwann Cells / cytology,  metabolism*
Sciatic Nerve / cytology,  injuries,  metabolism
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 0/S-100 calcium-binding protein beta subunit; 0/S100 Proteins; 103107-01-3/Fibroblast Growth Factor 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Oxidant/antioxidant balance in patients with thyroid cancer.
Next Document:  Reversibility of cataracts in diabetes.