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S100A9 promotes the proliferation and invasion of HepG2 hepatocellular carcinoma cells via the activation of the MAPK signaling pathway.
MedLine Citation:
PMID:  23354417     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The S100A9 protein, a member of the S100 protein family, is often upregulated in various types of cancer, including hepatocellular carcinoma (HCC). S100A9 acts as a danger signal when secreted to the extracellular space and is thought to play an important role during tumorigenesis. Despite this fact, little is known about the effects of S100A9 in the tumor microenvironment on HCC. Therefore, in this study, we investigated the effects of exogenous S100A9 on the proliferation and invasion of HepG2 HCC cells, as well as the molecular mechanisms underlying these effects. Our results demonstrated that exogenous S100A9 promoted the proliferation, clone formation and invasion of HepG2 cells in vitro, as shown by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltrazolium bromide (MTT), clone formation and transwell invasion assays, respectively, and also promoted tumor growth in vivo by tumorigenicity assays in nude mice. Furthermore, S100A9 increased the phosphorylation of p38 and ERK1/2 mitogen-activated protein kinases (MAPKs) in HepG2 cells. When the phosphorylation of p38 was inhibited by SB203580 (a p38 inhibitor), the S100A9-induced cell invasion was reversed; when the phosphorylation of ERK1/2 was inhibited by PD98059 (an ERK1/2 inhibitor), the S100A9-induced cell proliferation was reversed. These data suggest that the S100A9-induced proliferation and invasion of HepG2 cells are partly mediated by the activation of the MAPK signaling pathway.
Authors:
Rui Wu; Liang Duan; Liwei Ye; Haiyang Wang; Xia Yang; Yunyuan Zhang; Xian Chen; Yan Zhang; Yaguang Weng; Jingyong Luo; Min Tang; Qiong Shi; Tongchuan He; Lan Zhou
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Publication Detail:
Type:  Journal Article     Date:  2013-01-23
Journal Detail:
Title:  International journal of oncology     Volume:  42     ISSN:  1791-2423     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-01-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1001-10     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
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