Document Detail


S100A4 expression with reduced E-cadherin expression predicts distant metastasis of human malignant melanoma cell lines in the NOD/SCID/gammaCnull (NOG) mouse model.
MedLine Citation:
PMID:  16077966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tumor xenografts in immune-deficient mice (athymic nude mice and SCID mice) are well-established animal models for the study of human cancer. Several human melanoma cell lines were reported to metastasize in the immune-deficient mice models. However, metastatic rates were extremely low in spite of large numbers of injections of cancer cells, more than 1 x 10(6) cells/mouse. The NOD/SCID/gamma(C)(null) (NOG) mouse shows multiple immunological dysfunctions, including cytokine production capability, in addition to the functional incompetence of T, B and natural killer (NK) cells. However, the immune-deficient mice, with preserved NK cell activity, might interfere with engraftment efficiency. We examined the distant metastasis of the human melanoma cell lines (A2058, A375, G361 and HMY-1, 1 x 10(4) cells/mouse) in the 6 weeks after intravenous inoculation. All four melanoma cell lines showed metastasis in the NOG mice, while no metastatic lesions were observed in the NOD/SCID mice. Metastatic lesions were noted in the liver and lung of 6/6 (100%) mice at A2058, 8/9 (89%) at A375, 2/6 (33%) at G361 and 2/8 (25%) at HMY-1. A2058 and A375 cell lines with high metastatic potentials show increased gene expression of S100A4. Western blot assay confirmed the increased protein levels of S100A4 in the A2058 and A375 cell lines. E-cadherin gene expression was conversely inhibited in these cell lines. The increased expression of S100A4 combined with inhibited E-cadherin expression resulted in high metastatic potentials of the human melanoma cell lines in vivo.
Authors:
Norihiro Ikoma; Hitoshi Yamazaki; Yoshiyuki Abe; Yasuhisa Oida; Yasuyuki Ohnishi; Hiroshi Suemizu; Hozumi Matsumoto; Takashi Matsuyama; Yukinori Ohta; Akira Ozawa; Yoshito Ueyama; Masato Nakamura
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  14     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-03     Completed Date:  2005-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  633-7     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cadherins / genetics*,  metabolism
Cell Proliferation
Gene Expression Regulation, Neoplastic
Humans
Melanoma / genetics,  metabolism,  pathology*
Melanoma, Experimental / genetics,  metabolism,  pathology
Mice
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Neoplasm Metastasis
Neoplasm Transplantation
Predictive Value of Tests
Prognosis
RNA, Neoplasm / genetics,  metabolism
Receptors, Cytokine / genetics
Reverse Transcriptase Polymerase Chain Reaction
S100 Proteins / genetics*,  metabolism
Time Factors
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Cadherins; 0/RNA, Neoplasm; 0/Receptors, Cytokine; 0/S100 Proteins

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