| S100A10 regulates plasminogen-dependent macrophage invasion. | |
| | |
MedLine Citation:
|
PMID: 20424186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The plasminogen activation system plays an integral role in the migration of macrophages in response to an inflammatory stimulus, and the binding of plasminogen to its cell-surface receptor initiates this process. Although previous studies from our laboratory have shown the importance of the plasminogen receptor S100A10 in cancer cell plasmin production, the potential role of this protein in macrophage migration has not been investigated. Using thioglycollate to induce a peritoneal inflammatory response, we demonstrate, for the first time, that compared with wild-type (WT) mice, macrophage migration across the peritoneal membrane into the peritoneal cavity in S100A10-deficient (S100A10(-/-)) mice was decreased by up to 53% at 24, 48, and 72 hours. Furthermore, the number of S100A10-deficient macrophages that infiltrated Matrigel plugs was reduced by 8-fold compared with their WT counterpart in vivo. Compared with WT macrophages, macrophages from S100A10(-/-) mice demonstrated a 50% reduction in plasmin-dependent invasion across a Matrigel barrier and a 45% reduction in plasmin generation in vitro. This loss in plasmin-dependent invasion was in part the result of a decreased generation of plasmin and a decreased activation of pro-MMP-9 by S100A10-deficient macrophages. This study establishes a direct involvement of S100A10 in macrophage recruitment in response to inflammatory stimuli. |
| | |
Authors:
|
Paul A O'Connell; Alexi P Surette; Robert S Liwski; Per Svenningsson; David M Waisman |
Related Documents
:
|
7478126 - Effect of interferon-gamma in dialysis fluid on peritoneal defence in rats. 21115716 - Role of defensins in corneal epithelial barrier function against pseudomonas aeruginosa... 4085676 - Non-immune non-activated chicken macrophages destroy murine fibroblasts. 11471666 - Secondary peritonitis: severity of disease and activation of peritoneal cells. 7478126 - Effect of interferon-gamma in dialysis fluid on peritoneal defence in rats. 11006136 - A review of angiogenesis and antiangiogenic therapy with thalidomide in multiple myeloma. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-27 |
Journal Detail:
|
Title: Blood Volume: 116 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-20 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
|
Languages: eng Pagination: 1136-46 Citation Subset: AIM; IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Annexin A2 / physiology* Apoptosis Blotting, Western Cell Adhesion Cell Movement Cell Proliferation Collagen / metabolism Drug Combinations Female Fibrinolysin / metabolism Flow Cytometry Immunoenzyme Techniques Inflammation / chemically induced, metabolism, pathology* Laminin / metabolism Macrophages, Peritoneal / metabolism* Male Mice Mice, Inbred C57BL Mice, Knockout Plasminogen / metabolism* Proteoglycans / metabolism S100 Proteins / physiology* Thioglycolates / toxicity |
| Grant Support | |
ID/Acronym/Agency:
|
//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A2; 0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 0/S100 Proteins; 0/S100 calcium binding protein A10; 0/Thioglycolates; 119978-18-6/matrigel; 9001-91-6/Plasminogen; 9007-34-5/Collagen; EC 3.4.21.7/Fibrinolysin |
| Comments/Corrections | |
Comment In:
|
Blood. 2010 Aug 19;116(7):1022-4
[PMID:
20724548
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Macrophage-specific gene functions in Spi1-directed innate immunity.
Next Document: Clonal expansion and TCR-independent differentiation shape the HIV-specific CD8+ effector-memory T-c...