Document Detail


S100A1 regulates neurite organization, tubulin levels, and proliferation in PC12 cells.
MedLine Citation:
PMID:  9468532     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As a first step in determining what cellular processes are regulated by the calcium-modulated protein S100A1 isoform in neurons, the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examined. A mammalian expression vector containing the rat S100A1 cDNA in the antisense orientation with respect to a cytomegalovirus promoter was constructed and transfected into PC12 cells. Indirect immunofluorescence microscopy confirmed decreased S100A1 protein levels in all three stable transfectants (pAntisense clones) that expressed exogenous S100A1 antisense mRNA. In response to nerve growth factor, pAntisense clones extended significantly more neurites than control cells (4.01 +/- 0.16 versus 2.93 +/- 0.16 neurites/cell). This increase in neurite number was accompanied by an increase in total alpha-tubulin levels in untreated (4.0 +/- 0.6 versus 1.76 +/- 0.4 ng of alpha-tubulin/mg of total protein) and nerve growth factor-treated pAntisense clones (4.15 +/- 0.4 versus 2. 04 +/- 0.5 ng of alpha-tubulin/mg of total protein) when compared with control cells. At high cell densities, pAntisense clones exhibited a significant decrease in anchorage-dependent growth. In soft agar, pAntisense clones formed significantly more colonies (153 +/- 8%) than control cells (116 +/- 5%). However, the pAntisense soft agar colonies were significantly smaller than those observed in control cells (40.6 +/- 3.0 versus 59.5 +/- 1.2 micron). These data suggest that cell density inhibits both anchorage-independent and -dependent growth of pAntisense clones. In summary, ablation of S100A1 expression in PC12 cells results in increased tubulin levels, altered neurite organization, and decreased cell growth. Thus, S100A1 may directly link the cytoskeleton and calcium signal transduction pathways to cell proliferation.
Authors:
D B Zimmer; E H Cornwall; P D Reynolds; C M Donald
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  273     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-19     Completed Date:  1998-03-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4705-11     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, School of Medicine, University of South Alabama, Mobile, Alabama 36688, USA. dzimmer@jaguar1.usouthal.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Calcium-Binding Proteins / metabolism,  physiology*
Cell Division / physiology*
Cloning, Molecular
Fluorescent Antibody Technique, Indirect
Neurites*
Oligonucleotides, Antisense / genetics
PC12 Cells
Rats
Recombinant Proteins / metabolism
S100 Proteins
Signal Transduction
Subcellular Fractions / metabolism
Tubulin / metabolism*
Grant Support
ID/Acronym/Agency:
NS 30660/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Oligonucleotides, Antisense; 0/Recombinant Proteins; 0/S100 Proteins; 0/S100A1 protein; 0/Tubulin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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