Document Detail


An S1 nuclease-sensitive homopurine/homopyrimidine domain in the PDGF A-chain promoter contains a novel binding site for the growth factor-inducible protein EGR-1.
MedLine Citation:
PMID:  1417865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transcription of the platelet-derived growth factor (PDGF) A-chain gene is activated in cells exposed to growth factors. We now have identified a homopurine/homopyrimidine domain in the promoter region of the PDGF A-chain gene that exhibits S1 nuclease sensitivity in vitro and that contains a novel binding site (5'-TCCTCCTCCTCCTC-3') for the growth factor inducible transcription factor EGR-1 as demonstrated in gel mobility shift assays. Sequences similar to this novel EGR-1 binding site were observed also in five growth-related genes and shown to bind to EGR-1 in competition assays, suggesting that EGR-1 may influence the transcriptional regulation of these growth-related genes.
Authors:
Z Y Wang; T F Deuel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  188     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-11-19     Completed Date:  1992-11-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  433-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites
DNA-Binding Proteins / biosynthesis,  metabolism*
Growth Substances / pharmacology
Macromolecular Substances
Molecular Sequence Data
Oligodeoxyribonucleotides
Platelet-Derived Growth Factor / genetics*
Promoter Regions, Genetic*
Sequence Homology, Nucleic Acid
Single-Strand Specific DNA and RNA Endonucleases / metabolism*
Substrate Specificity
Transcription Factors / biosynthesis,  metabolism*
Grant Support
ID/Acronym/Agency:
CA49712/CA/NCI NIH HHS; HL14147/HL/NHLBI NIH HHS; HL31102/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Growth Substances; 0/Macromolecular Substances; 0/Oligodeoxyribonucleotides; 0/Platelet-Derived Growth Factor; 0/Transcription Factors; EC 3.1.30.1/Single-Strand Specific DNA and RNA Endonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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