| S-nitrosoglutathione-induced toxicity in Drosophila melanogaster: Delayed pupation and induced mild oxidative/nitrosative stress in eclosed flies. | |
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MedLine Citation:
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PMID: 22940110 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The toxicity of the nitric oxide donor S-nitrosoglutathione (GSNO) was tested on the Drosophila melanogaster model system. Fly larvae were raised on food supplemented with GSNO at concentrations of 1.0, 1.5 or 4.0mM. Food supplementation with GSNO caused a developmental delay in the flies. Biochemical analyses of oxidative stress markers and activities of antioxidant and associated enzymes were carried out on 2-day-old flies that emerged from control larvae and larvae fed on food supplemented with GSNO. Larval exposure to GSNO resulted in lower activities of aconitase in both sexes and also lower activities of catalase and isocitrate dehydrogenase in adult males relative to the control cohort. Larval treatment with GSNO resulted in higher carbonyl protein content and higher activities of glucose-6-phosphate dehydrogenase in males and higher activities of superoxide dismutase and glutathione-S-transferase in both sexes. Among the parameters tested, aconitase activity and developmental end points may be useful early indicators of toxicity caused by GSNO. |
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Authors:
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Oleksandr V Lozinsky; Oleh V Lushchak; Natalia I Kryshchuk; Natalia Y Shchypanska; Anna H Riabkina; Stanislava V Skarbek; Ivan V Maksymiv; Janet M Storey; Kenneth B Storey; Volodymyr I Lushchak |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-25 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Volume: - ISSN: 1531-4332 ISO Abbreviation: Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-9-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806096 Medline TA: Comp Biochem Physiol A Mol Integr Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Department of Biochemistry and Biotechnology, Precarpathian National University named after Vassyl Stefanyk, 57 Shevchenko Str., Ivano-Frankivsk 76025, Ukraine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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