| S-deficiency responsive accumulation of amino acids is mainly due to hydrolysis of the previously synthesized proteins - not to de novo synthesis in Brassica napus. | |
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MedLine Citation:
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PMID: 22725131 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To characterize the mechanisms of amino acid accumulation under sulphur deficiency and its physiological significance in Brassica napus, stable isotopes (15) N and (34) S were employed. The plants were exposed for 9 days to S-deficient conditions (0.05 mM vs. 1.5 mM sulphate). After 9 d of S-deficiency, leaf-osmotic potential and total chlorophyll content significantly decreased. S uptake decreased by 94%, whereas N uptake and biomass were not significantly changed. Using (15) N and (34) S labeling, de novosynthesis of amino acids and proteins derived from newly absorbed NO(3) (-) and SO(4) (2) (-) and the content of N and S inthe previously synthesized amino acids and proteins were quantified. At the whole plant level, S-deficiency increased the pool of amino acids but resulted in strong decrease of incorporation of newly absorbed NO(3) (-) and SO(4) (2) (-) into amino acids by 22.2% and 76.6%, respectively, compared to the controls. Total amount of N and S incorporated into proteins also decreased by 28.8% and 62.1%,respectively. The levels of(14) N- and (32) S-proteins (previously synthesized proteins) strongly decreased, mainly in mature leaves. The data thus indicate that amino acid accumulation under short term S-deficiency results from the degradation of previously synthesized proteins rather than from de novo synthesis. |
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Authors:
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Bok-Rye Lee; Sowbiya Muneer; Kil-Yong Kim; Jean-Christophe Avice; Alain Ourry; Tae-Hwan Kim |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-22 |
Journal Detail:
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Title: Physiologia plantarum Volume: 9999 ISSN: 1399-3054 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1256322 Medline TA: Physiol Plant Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © Physiologia Plantarum 2012. |
Affiliation:
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Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju, 500-600, Korea; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824-1319, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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