Document Detail


S-deficiency responsive accumulation of amino acids is mainly due to hydrolysis of the previously synthesized proteins - not to de novo synthesis in Brassica napus.
MedLine Citation:
PMID:  22725131     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To characterize the mechanisms of amino acid accumulation under sulphur deficiency and its physiological significance in Brassica napus, stable isotopes (15) N and (34) S were employed. The plants were exposed for 9 days to S-deficient conditions (0.05 mM vs. 1.5 mM sulphate). After 9 d of S-deficiency, leaf-osmotic potential and total chlorophyll content significantly decreased. S uptake decreased by 94%, whereas N uptake and biomass were not significantly changed. Using (15) N and (34) S labeling, de novosynthesis of amino acids and proteins derived from newly absorbed NO(3) (-) and SO(4) (2) (-) and the content of N and S inthe previously synthesized amino acids and proteins were quantified. At the whole plant level, S-deficiency increased the pool of amino acids but resulted in strong decrease of incorporation of newly absorbed NO(3) (-) and SO(4) (2) (-) into amino acids by 22.2% and 76.6%, respectively, compared to the controls. Total amount of N and S incorporated into proteins also decreased by 28.8% and 62.1%,respectively. The levels of(14) N- and (32) S-proteins (previously synthesized proteins) strongly decreased, mainly in mature leaves. The data thus indicate that amino acid accumulation under short term S-deficiency results from the degradation of previously synthesized proteins rather than from de novo synthesis.
Authors:
Bok-Rye Lee; Sowbiya Muneer; Kil-Yong Kim; Jean-Christophe Avice; Alain Ourry; Tae-Hwan Kim
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-22
Journal Detail:
Title:  Physiologia plantarum     Volume:  9999     ISSN:  1399-3054     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © Physiologia Plantarum 2012.
Affiliation:
Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju, 500-600, Korea; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824-1319, USA.
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