| The Ryk Receptor Is Expressed in Glial and Fibronectin-Expressing Cells after Spinal Cord Injury. | |
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MedLine Citation:
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PMID: 23320533 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Wnt proteins play a critical role in central nervous system development and have been implicated in several neuropathologies, including spinal cord injury (SCI). Ryk, a non-conventional Wnt receptor, regulates axonal regeneration after SCI, although its expression pattern in this neuropathology remains unclear. Thus, we sought to define the spatio-temporal and cellular pattern of Ryk expression after a contusive SCI in adult rats using quantitative RT-PCR, western blot and immunohistochemical analysis. Under physiological conditions, Ryk is expressed in neurons, astrocytes, and blood vessels, but not in oligodendrocytes, microglia, NG2+ glial precursor cells or axonal projections. Following SCI, we observed an increase in Ryk mRNA expression from 24 hours post-injury until 7 days post-injury, while its protein levels were significantly augmented at 7 and 14 days post-injury. Moreover, the spatial and cellular Ryk expression pattern was altered in the damaged tissue, where this receptor was observed in reactive astrocytes and microglia/macrophages, NG2+ glial precursors, fibronectin+ cells, oligodendrocytes and axons. In conclusion, we demonstrate that Ryk is expressed in the non-lesioned spinal cord and that, after SCI, its spatio-temporal and cellular expression pattern changed dramatically, being expressed in cells involved in the spinal cord response to damage. Keywords: glia cell response to injury, receptors, traumatic spinal cord injury, in vivo studies, rat. |
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Authors:
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Pau González; Carmen María Fernandez-Martos; Ernest Arenas; F Javier Rodríguez |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-16 |
Journal Detail:
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Title: Journal of neurotrauma Volume: - ISSN: 1557-9042 ISO Abbreviation: J. Neurotrauma Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8811626 Medline TA: J Neurotrauma Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Hospital Nacional de Parapléjicos, Group of Molecular Neurology, Toledo, Spain; paug@sescam.jccm.es. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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