Document Detail

The Ryk Receptor Is Expressed in Glial and Fibronectin-Expressing Cells after Spinal Cord Injury.
MedLine Citation:
PMID:  23320533     Owner:  NLM     Status:  Publisher    
Wnt proteins play a critical role in central nervous system development and have been implicated in several neuropathologies, including spinal cord injury (SCI). Ryk, a non-conventional Wnt receptor, regulates axonal regeneration after SCI, although its expression pattern in this neuropathology remains unclear. Thus, we sought to define the spatio-temporal and cellular pattern of Ryk expression after a contusive SCI in adult rats using quantitative RT-PCR, western blot and immunohistochemical analysis. Under physiological conditions, Ryk is expressed in neurons, astrocytes, and blood vessels, but not in oligodendrocytes, microglia, NG2+ glial precursor cells or axonal projections. Following SCI, we observed an increase in Ryk mRNA expression from 24 hours post-injury until 7 days post-injury, while its protein levels were significantly augmented at 7 and 14 days post-injury. Moreover, the spatial and cellular Ryk expression pattern was altered in the damaged tissue, where this receptor was observed in reactive astrocytes and microglia/macrophages, NG2+ glial precursors, fibronectin+ cells, oligodendrocytes and axons. In conclusion, we demonstrate that Ryk is expressed in the non-lesioned spinal cord and that, after SCI, its spatio-temporal and cellular expression pattern changed dramatically, being expressed in cells involved in the spinal cord response to damage. Keywords: glia cell response to injury, receptors, traumatic spinal cord injury, in vivo studies, rat.
Pau González; Carmen María Fernandez-Martos; Ernest Arenas; F Javier Rodríguez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Journal of neurotrauma     Volume:  -     ISSN:  1557-9042     ISO Abbreviation:  J. Neurotrauma     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8811626     Medline TA:  J Neurotrauma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Hospital Nacional de Parapléjicos, Group of Molecular Neurology, Toledo, Spain;
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