Document Detail

The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury.
MedLine Citation:
PMID:  23320533     Owner:  NLM     Status:  MEDLINE    
Wnt proteins play a critical role in central nervous system development and have been implicated in several neuropathologies, including spinal cord injury (SCI). Ryk, an unconventional Wnt receptor, regulates axonal regeneration after SCI, although its expression pattern in this neuropathology remains unclear. Therefore, we sought to define the spatiotemporal and cellular pattern of Ryk expression after a contusive SCI in adult rats using quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemical analysis. Under physiological conditions, Ryk is expressed in neurons, astrocytes, and blood vessels, but not in oligodendrocytes, microglia, NG2+ glial precursor cells, or axonal projections. Following SCI, we observed an increase in Ryk mRNA expression from 24 h post-injury until 7 days post-injury, whereas its protein levels were significantly augmented at 7 and 14 days post-injury. Moreover, the spatial and cellular Ryk expression pattern was altered in the damaged tissue, where this receptor was observed in reactive astrocytes and microglia/macrophages, NG2+ glial precursors, fibronectin+ cells, oligodendrocytes, and axons. In conclusion, we demonstrate that Ryk is expressed in the unlesioned spinal cord and that, after SCI, its spatiotemporal and cellular expression pattern changed dramatically, being expressed in cells involved in the spinal cord response to damage.
Pau González; Carmen María Fernández-Martos; Ernest Arenas; Francisco Javier Rodríguez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-05-09
Journal Detail:
Title:  Journal of neurotrauma     Volume:  30     ISSN:  1557-9042     ISO Abbreviation:  J. Neurotrauma     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-22     Completed Date:  2013-12-23     Revised Date:  2014-05-16    
Medline Journal Info:
Nlm Unique ID:  8811626     Medline TA:  J Neurotrauma     Country:  United States    
Other Details:
Languages:  eng     Pagination:  806-17     Citation Subset:  IM    
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MeSH Terms
Axons / metabolism,  pathology
Cells, Cultured
Fibronectins / metabolism*
Neuroglia / metabolism*,  pathology
Neurons / metabolism*,  pathology
Rats, Wistar
Receptor Protein-Tyrosine Kinases / genetics,  metabolism*
Spinal Cord / metabolism*,  pathology
Spinal Cord Injuries / metabolism*,  pathology
Reg. No./Substance:
0/Fibronectins; EC 2.7.1.-/Ryk protein, rat; EC Protein-Tyrosine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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