| Runx3 negatively regulates Osterix expression in dental pulp cells. | |
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MedLine Citation:
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PMID: 17352693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Osterix, a zinc-finger-containing transcription factor, is required for osteoblast differentiation and bone formation. Osterix is also expressed in dental mesenchymal cells of the tooth germ. However, transcriptional regulation by Osterix in tooth development is not clear. Genetic studies in osteogenesis place Osterix downstream of Runx2 (Runt-related 2). The expression of Osterix in odontoblasts overlaps with Runx3 during terminal differentiation in vivo. Runx3 down-regulates Osterix expression in mouse DPCs (dental pulp cells). Therefore the regulatory role of Runx3 on Osterix expression in tooth development was investigated. Enforced expression of Runx3 down-regulated the activity of the Osterix promoter in the human embryonic kidney 293 cell line. When the Runx3 responsive element on the Osterix promoter, located at -713 to -707 bp (site 3, AGTGGTT) relative to the cap site, was mutated, this down-regulation was abrogated. Furthermore, electrophoretic mobility-shift assay and chromatin immunoprecipitation assays in mouse DPCs demonstrated direct functional binding of Runx3 to the Osterix promoter. These results demonstrate the transcriptional regulation of Osterix expression by Runx3 during differentiation of dental pulp cells into odontoblasts during tooth development. |
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Authors:
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Li Zheng; Koichiro Iohara; Masaki Ishikawa; Takeshi Into; Teruko Takano-Yamamoto; Kenji Matsushita; Misako Nakashima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 405 ISSN: 1470-8728 ISO Abbreviation: Biochem. J. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-08 Completed Date: 2007-07-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 69-75 Citation Subset: IM |
Affiliation:
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Laboratory of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Aichi 474-8522, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / genetics, metabolism Cell Differentiation / physiology* Cell Line Core Binding Factor Alpha 1 Subunit / genetics, metabolism Core Binding Factor Alpha 3 Subunit / genetics, metabolism* Dental Pulp / cytology*, physiology Gene Expression Regulation, Developmental* Humans In Situ Hybridization Mice Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic Protein Binding Transcription Factors / genetics, metabolism* Transforming Growth Factor beta / genetics, metabolism Zinc Fingers |
| Chemical | |
Reg. No./Substance:
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0/BMP2 protein, human; 0/Bmp2 protein, mouse; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/Core Binding Factor Alpha 1 Subunit; 0/Core Binding Factor Alpha 3 Subunit; 0/Runx2 protein, mouse; 0/Runx3 protein, mouse; 0/Transcription Factors; 0/Transforming Growth Factor beta; 0/osterix protein, mouse |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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