Document Detail


Ruminal infusions of cobalt-EDTA reduce mammary delta9-desaturase index and alter milk fatty acid composition in lactating cows.
MedLine Citation:
PMID:  18356325     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ruminal administration of a triple indigestible marker system comprised of cobalt EDTA (CoEDTA), ytterbium acetate (YbAc), and chromium-mordanted straw (CrS) decreases product:substrate ratios for Delta9-desaturase in bovine milk fat. This experiment was designed to identify the marker(s) responsible and develop an alternative system for simultaneous determination of nutrient flow in the gastro-intestinal tract and milk fatty acid composition. Five lactating dairy cows were used in a 5 x 5 Latin square with 21-d periods to evaluate the effects of YbAc, CoEDTA, and CrS independently or as part of a triple marker system (TMS), and CrEDTA as an alternative to CoEDTA on milk fat composition. Markers were administered in the rumen over a 7-d interval and samples of milk were collected on d -1, 3, 7, and 11. Both TMS and CoEDTA alone reduced the concentrations of milk fatty acids containing a cis-9 double bond, whereas YbAc, CrS, and CrEDTA had no effect. Reductions in product:substrate ratios for Delta9-desaturase were time dependent and evident within 3 d of administration. Ruminal infusion of CoEDTA for 7 d induced mean decreases in milk cis-9 14:1/14:0, cis-9 16:1/16:0, cis-9 18:1/18:0, and cis-9, trans-11 conjugated linoleic acid/trans-11 18:1 concentration ratios of 47.7, 26.7, 40.3, and 42.6%, respectively. In conclusion, ruminal infusion of CoEDTA alters milk fatty acid composition and appears to inhibit Delta9-desaturase activity in the bovine mammary gland. Results indicate that a TMS based on CrEDTA, YbAc, and indigestible neutral detergent fiber can be used for estimating nutrient flow without altering milk fat composition in lactating cows.
Authors:
Kevin J Shingfield; Anu Arölä; Seppo Ahvenjärvi; Aila Vanhatalo; Vesa Toivonen; J Mikko Griinari; Pekka Huhtanen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  138     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-21     Completed Date:  2008-04-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  710-7     Citation Subset:  IM    
Affiliation:
Animal Production Research, MTT Agrifood Research Finland, Jokioinen, FIN 31600, Finland. kevin.shingfield@mtt.fi
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animal Nutritional Physiological Phenomena
Animals
Biological Markers
Cattle / metabolism*
Chromium / administration & dosage,  pharmacology,  urine
Edetic Acid / administration & dosage,  pharmacology*,  urine
Fatty Acids / analysis*
Female
Lactation
Mammary Glands, Animal / enzymology*
Milk / chemistry*
Rumen / metabolism*
Stearoyl-CoA Desaturase / metabolism*
Time Factors
Ytterbium / administration & dosage,  pharmacology,  urine
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids; 14931-83-0/cobalt-ethylenediamine tetraacetic acid chelate; 60-00-4/Edetic Acid; 7440-47-3/Chromium; 7440-64-4/Ytterbium; EC 1.14.19.1/Stearoyl-CoA Desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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