| Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts. | |
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MedLine Citation:
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PMID: 19583786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Within this study, we investigated whether the polyunsaturated fatty acids (PUFA)-rich nature of rumen protozoa is a consequence of ingestion of PUFA-rich chloroplasts. Four Hereford x Friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perennial ryegrass [high 18:3 (n-3) and high chlorophyll concentration] for 16 days. On the 14th and 16th days, rumen protozoa as well as attached and planktonic bacteria were fractionated 1 h before (-1 h), 2 and 6 h postfeeding, and their fatty acid concentrations determined. Protozoa fractionated from fresh grass-fed steers were richer (P<0.05) in PUFA, except conjugated linoleic acid, for all time points compared with those from hay-fed steers. Protozoal density was higher (P<0.05) for grass compared with hay. Entodinomorphid abundance was 3.4 times higher on fresh grass (P<0.01) compared with hay. Confocal microscopy and transmission electron microscopy confirmed that Epidinium spp. were commonly saturated with intracellular cytoplasmic chloroplasts. These data suggest that engulfment of chloroplasts is a major contributor to the high 18:3 (n-3) concentration of protozoa. |
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Authors:
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Sharon A Huws; Eun J Kim; Alison H Kingston-Smith; Michael R F Lee; Stefan M Muetzel; Alan R Cookson; Charles J Newbold; R John Wallace; Nigel D Scollan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-05 |
Journal Detail:
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Title: FEMS microbiology ecology Volume: 69 ISSN: 1574-6941 ISO Abbreviation: FEMS Microbiol. Ecol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-18 Completed Date: 2009-09-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8901229 Medline TA: FEMS Microbiol Ecol Country: England |
Other Details:
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Languages: eng Pagination: 461-71 Citation Subset: IM |
Affiliation:
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Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth SY23 3EB, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis Animals Bacteria / genetics, metabolism Cattle Chlorophyll / metabolism Chloroplasts / metabolism* DNA, Bacterial / genetics DNA, Protozoan / genetics Eukaryota / genetics, metabolism* Fatty Acids, Unsaturated / metabolism* Male RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 18S / genetics Rumen / microbiology* |
| Grant Support | |
ID/Acronym/Agency:
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//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Protozoan; 0/Fatty Acids, Unsaturated; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 18S; 1406-65-1/Chlorophyll |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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