Document Detail


Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts.
MedLine Citation:
PMID:  19583786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Within this study, we investigated whether the polyunsaturated fatty acids (PUFA)-rich nature of rumen protozoa is a consequence of ingestion of PUFA-rich chloroplasts. Four Hereford x Friesian steers were offered hay [low 18:3 (n-3) and low chlorophyll concentration] followed by freshly cut perennial ryegrass [high 18:3 (n-3) and high chlorophyll concentration] for 16 days. On the 14th and 16th days, rumen protozoa as well as attached and planktonic bacteria were fractionated 1 h before (-1 h), 2 and 6 h postfeeding, and their fatty acid concentrations determined. Protozoa fractionated from fresh grass-fed steers were richer (P<0.05) in PUFA, except conjugated linoleic acid, for all time points compared with those from hay-fed steers. Protozoal density was higher (P<0.05) for grass compared with hay. Entodinomorphid abundance was 3.4 times higher on fresh grass (P<0.01) compared with hay. Confocal microscopy and transmission electron microscopy confirmed that Epidinium spp. were commonly saturated with intracellular cytoplasmic chloroplasts. These data suggest that engulfment of chloroplasts is a major contributor to the high 18:3 (n-3) concentration of protozoa.
Authors:
Sharon A Huws; Eun J Kim; Alison H Kingston-Smith; Michael R F Lee; Stefan M Muetzel; Alan R Cookson; Charles J Newbold; R John Wallace; Nigel D Scollan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-05
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  69     ISSN:  1574-6941     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-09-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-71     Citation Subset:  IM    
Affiliation:
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth SY23 3EB, UK.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis
Animals
Bacteria / genetics,  metabolism
Cattle
Chlorophyll / metabolism
Chloroplasts / metabolism*
DNA, Bacterial / genetics
DNA, Protozoan / genetics
Eukaryota / genetics,  metabolism*
Fatty Acids, Unsaturated / metabolism*
Male
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 18S / genetics
Rumen / microbiology*
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Protozoan; 0/Fatty Acids, Unsaturated; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 18S; 1406-65-1/Chlorophyll

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