Document Detail


Rumen fermentation and metabolic profile in conventional and gnotobiotic lambs.
MedLine Citation:
PMID:  8585797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Observations were carried out of actual acidity, volatile fatty acid (VFA) concentrations, enzyme activity in the rumen, total protein, urea, total lipid and glucose in the serum of conventional (CL) and gnotobiotic lambs (GL) in the period of milk nutrition. The inoculum of gnotobiotic lambs contained Streptococcus bovis, Prevoxella ruminicola, Butyrivibrio fibrisolvens and Selenomonas ruminantium at a concentration of 1.10(6) each. Throughout the observation period the pH of the rumen contents of gnotobiotic lambs ranged within 6.5-6.8 with a significant difference at an age of 7 weeks. Total VFA concentrations in the rumen contents were increased in the CL throughout milk nutrition: the differences at 4 and 5 weeks of age were significant. Total VFA in the conventional lambs revealed an increasing tendency between weeks 4 and 7, reaching higher levels at 7 weeks of age (57.1 mmol.l-1), whereas in the gnotobiotic animals the range (24.3-30.1 mmol.l-1) was narrow and the peak occurred at 6 weeks of age. In GL significantly increased molar proportions of acetic acid were observed whereas in CL the molar proportions of propionic acid proved to be significant increased. The molar proportions of butyric and valeric acids were increased in CL but the group differences were not significant. In GL no isoacids were found. Alpha amylase (E.C.3.2.1.1.) activity of the rumen contents was significantly increased in GL between weeks 2 and 6 of age whereas cellulase (endoglucanase E.C.3.2.1.4. and cellobiohydrolase E.C.3.2.1.91.) activity was significantly increased in 4-week-old CL. Over the whole period of milk nutrition no significant differences were observed in urease (E.C.3.5.1.5.) activity of the rumen contents in the examined groups. At 5 weeks of age significantly increased total protein levels were observed in the conventional animals with maximum levels occurring at 4 weeks of age (CL-59.5 g.l-1 GL-55.3 g.l-1). Urea levels in 6-week old conventional lambs were significantly higher than in the gnotobiotic animals (CL-6.4 mmol.l-1 vs. GL-1.9 mmol.l-1). As to glycaemia no significant group differences were recorded. In the conventional animals total lipid levels were significantly increased at 1 and 6 weeks of age with a peak occurring in the first week of life (7.5 g.l-1) whereas in the gnotobiotic lambs a significant increase was observed at 3 weeks of age, the peak being recorded in 4 week-old animals (4.3 g.l-1). Throughout the period of interest the mean daily weight gains in the conventional and gnotobiotic lambs presented 0.164 and 0.162 kg, respectively.(ABSTRACT TRUNCATED AT 400 WORDS)
Authors:
A Bomba; R Zitnan; I Koniarová; A Lauková; A Sommer; J Posivák; V Bucko; J Pataky
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archiv für Tierernährung     Volume:  48     ISSN:  0003-942X     ISO Abbreviation:  Arch Tierernahr     Publication Date:  1995  
Date Detail:
Created Date:  1996-03-15     Completed Date:  1996-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217641     Medline TA:  Arch Tierernahr     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  231-43     Citation Subset:  IM    
Affiliation:
Research Institute of Experimental Veterinary Medicine, Kosice, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Blood Glucose / metabolism
Cellulase / metabolism
Fatty Acids, Volatile / analysis
Female
Fermentation
Gastrointestinal Contents / chemistry
Germ-Free Life*
Hydrogen-Ion Concentration
Lipids / blood
Milk
Reference Values
Rumen / physiology*
Sheep / physiology*
Weight Gain
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids, Volatile; 0/Lipids; EC 3.2.1.4/Cellulase

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