Document Detail

Ruffling and locomotion: role in cell resistance to growth factor-induced proliferation.
MedLine Citation:
PMID:  8436604     Owner:  NLM     Status:  MEDLINE    
It has long been known that the growth rate of cells in vitro can be retarded by providing substrates of restricted area. Such experiments were performed with adhesive islets, made by depositing metals onto agarose layers through templates of various sizes. Since normal cells are unable to adhere to agarose, they become confined to the metallic surface. Using such haptotactic islets, we have studied the role of membrane ruffling and cell locomotion in the resistance of AG1523 human fibroblasts to growth factor-induced mitogenesis. Cells plated on small substrates, i.e., 2,150 microns 2 in area, initially showed vigorous ruffling, which was suppressed by 8 h after plating but had resumed again by 12 h. In contrast, cells on larger-size islets showed a rapid decline and stabilization of ruffling activity. When the growth rate was measured for single cells cultured on haptotactic islets, it was found to increase linearly from areas of 4,280 microns 2 up to 425,000 microns 2. Since the area needed to saturate the growth response was approximately 50-fold larger than the area occupied by a single cell, the growth inhibition was attributed in part to an interference with locomotion. The implication that locomotion provided positive input into growth control mechanisms was subjected to a direct test by evaluating the effect of nine polypeptide growth factors on the motility of serum-starved cells. All except TGF-beta 1 stimulated movement. Finally, the mitogenic effect of growth factors was measured by [3H]thymidine incorporation and found to be proportional to motile activities, as quantitatively assayed. We conclude that locomotion suppression is a factor in AG1523 cell resistance to growth factor-induced mitogenesis.
C A Heckman; K I Oravecz; D Schwab; J Pontén
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  154     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-03-23     Completed Date:  1993-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  554-65     Citation Subset:  IM    
Department of Biological Sciences, Bowling Green State University, Ohio 43403.
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MeSH Terms
Cell Division*
Cell Line
Cell Membrane / ultrastructure*
Cell Movement* / drug effects
Cell Size*
DNA / biosynthesis
Growth Substances / pharmacology*
Microscopy, Electron, Scanning
S Phase
Reg. No./Substance:
0/Growth Substances; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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