| Rubidium release: a rapid and sensitive assay for amphotericin B. | |
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MedLine Citation:
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PMID: 789783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new assay for amphotericin B has been devised with use of the release of a radioactive potassium analogue (86rubidium; 86Rb) from Candida parapsilosis. The 86Rb-release assay is rapid, highly sensitive, and reproducible. Amphotericin B levels of 0.06-2.0 mug/ml can be measured in serum, whereas as little as 0.015 mug of amphotericin B/ml can be detected in spinal fluid. The greater sensitivity of the assay in spinal fluid as compared with that in serum may be the result of binding of the amphotericin molecules by serum components, thus decreasing the amount of amphotericin free to interact with the fungal membrane. |
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Authors:
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R E Drazin; R I Lehrer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 134 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 1976 Sep |
Date Detail:
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Created Date: 1976-12-23 Completed Date: 1976-12-23 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 238-44 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amphotericin B
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analysis*,
blood,
cerebrospinal fluid Biological Assay Candida / metabolism* Candida albicans / metabolism Kinetics Radioisotopes Rubidium / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Radioisotopes; 1397-89-3/Amphotericin B; 7440-17-7/Rubidium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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