| Routing of internalized ricin and ricin conjugates to the Golgi complex. | |
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MedLine Citation:
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PMID: 3001103 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Receptor-mediated endocytosis and intracellular routing of native ricin, and of ricin conjugated to colloidal gold (Ri-Au) and to horseradish peroxidase (Ri-HRP), have been studied in cultured MCF-7 and Vero cells by electron microscopical techniques including serial section analysis. Both native ricin, as demonstrated by immunoperoxidase cytochemistry, and the ricin conjugates were internalized via a common coated pit-coated vesicle pathway to reach vacuolar and tubulo-vesicular portions of the endosomal system. In addition, native ricin and a purified monovalent fraction of Ri-HRP reached distinct Golgi cisterns, whereas Ri-Au and polyvalent Ri-HRP did not. The results delineate intracellular routing of native ricin and compare it with the routing of different ricin conjugates. Moreover, our study shows that conjugates of a particular ligand (ricin) and various probes (e.g., gold and peroxidase), may be handled differently by cells. Sorting apparently takes place in the endosomal system, allowing some but not other molecules to reach Golgi elements. This sorting seems to depend on the valency of the ricin conjugate. |
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Authors:
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B van Deurs; T I Tønnessen; O W Petersen; K Sandvig; S Olsnes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 102 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-02-19 Completed Date: 1986-02-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 37-47 Citation Subset: IM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Breast Neoplasms Cell Line Endocytosis Female Ferritins / metabolism Gold / metabolism Golgi Apparatus / metabolism* Horseradish Peroxidase / metabolism Humans Immunologic Techniques Macromolecular Substances Microscopy, Electron Receptors, Cell Surface / metabolism Ricin / metabolism* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 0/Receptors, Cell Surface; 0/polycationic ferritin; 7440-57-5/Gold; 9007-73-2/Ferritins; 9009-86-3/Ricin; EC 1.11.1.-/Horseradish Peroxidase |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 1 Year: 1986 Volume: 102 Issue: 1 First Page: 37 Last Page: 47 ID: 2114043 Publisher Id: 86086135 PubMed Id: 3001103 |
| Routing of internalized ricin and ricin conjugates to the Golgi complex | |
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