Document Detail


Routine heat inactivation of serum reduces its capacity to promote cell attachment.
MedLine Citation:
PMID:  3667489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heat inactivation (56 degrees C for 40 min) of bovine calf serum was shown to diminish its capacity to promote the attachment of cells to plastic or glass surfaces. This effect was not observed in stationary cultures (culture dishes) but became manifest under conditions in which the cells were subjected to a small amount of liquid shear force, i.e. by growing cells in roller bottles or culture tubes. Of four cell lines tested on bovine calf serum (SV-BHK, BALB-3T3, CV-1, and FS-4) SV-BHK and CV-1 cells showed the greatest sensitivity to loss of attachment-promoting activity. Fetal bovine serum also seemed to be affected by heat inactivation but to a lesser degree than bovine calf serum. Treatment of vessel surfaces with either unheated calf serum or specific attachment factors (gelatin, poly-D-lysine, and fibronectin) greatly increased cell attachment in the presence of heat inactivated serum. Heat inactivation did not seem to affect the ability of cells to grow after attachment. Of the four cell lines tested, the normal human fibroblast line (FS-4) was shown to be most effective at conditioning medium and restoring its capacity to promote the attachment of all four cell lines.
Authors:
D J Giard
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  23     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1987-12-04     Completed Date:  1987-12-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  691-7     Citation Subset:  IM    
Affiliation:
Cell Culture Center, Massachusetts Institute of Technology, Cambridge 02139.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Physiological Phenomena*
Cattle
Cell Adhesion*
Cell Line
Cercopithecus aethiops
Cricetinae
Culture Media / pharmacology*
Culture Techniques / instrumentation
Fibroblasts / physiology*
Glass
Hot Temperature*
Kidney
Mesocricetus
Mice
Mice, Inbred BALB C
Plastics
Grant Support
ID/Acronym/Agency:
SRC 5 U24 RR02557-02/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Plastics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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