Document Detail

Routine growth of cell lines in medium supplemented with milk instead of serum.
MedLine Citation:
PMID:  7226249     Owner:  NLM     Status:  MEDLINE    
Several cell lines can be grown in media, supplemented with milk instead of serum. To obtain a good plating efficiency the medium containing milk must be supplemented with calf serum at a final concentration of 0.5%. The milk must be centrifuged before use to get rid of the fat and cellular debris. Using these precautions several monolayer cell lines can be grown routinely in milk supplemented media, just as well as in serum supplemented media. The economic advantages of using milk instead of serum are considerable.
A Sereni; R Baserga
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell biology international reports     Volume:  5     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-07-09     Completed Date:  1981-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  339-45     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cell Line
Cells, Cultured / physiology*
Culture Media*
DNA / biosynthesis
Grant Support
Reg. No./Substance:
0/Culture Media; 9007-49-2/DNA

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