| Route of jejunal mucosa absorption of trypsin demonstrated by immunofluorescence. | |
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MedLine Citation:
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PMID: 14626341 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have demonstrated the absorption of porcine trypsin in isolated jejunal loops from male Wistar rats by open-loop perfusion. The possible routes of absorption were examined in the study reported here. Trypsin (0.5 mg/ml) was dissolved in tyrode solution and perfused at a rate of 0.5 ml/min, at 37 degrees C, for 40 min. Using immunoperoxidase and immunofluorescence techniques, strong reactivity towards anti-TLCK-trypsin antibody was demonstrated through out the enterocyte cytosol. The present data indicate that trypsin was absorbed by enterocytes, probably through a transcellular route. |
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Authors:
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Elizabeth L Borges; Maria de Fátima Leite; Alfredo J A Barbosa; José B Alves |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 34 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 2002 |
Date Detail:
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Created Date: 2003-11-19 Completed Date: 2004-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: Netherlands |
Other Details:
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Languages: eng Pagination: 525-8 Citation Subset: IM |
Affiliation:
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Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fluorescent Antibody Technique, Indirect Immunoenzyme Techniques Intestinal Absorption / physiology* Intestinal Mucosa / metabolism* Jejunum / metabolism* Male Perfusion Rats Rats, Wistar Trypsin / metabolism* |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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