Document Detail

Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study.
MedLine Citation:
PMID:  16684848     Owner:  NLM     Status:  MEDLINE    
A pathway from enteral L-glutamine as substrate for L-arginine synthesis is suggested by previous studies. L-Glutamine and L-glutamine dipeptides exhibit numerous beneficial effects in experimental and clinical studies. In trauma patients, enteral L-glutamine supply increased plasma L-arginine. The present study was designed to quantify the contribution of L-glutamine to the de novo L-citrulline and L-arginine synthesis in mice when L-glutamine is administered in a high dose of labeled L-glutamine or L-alanyl-L-glutamine by the enteral or parenteral route. For this purpose, male Swiss mice (n = 43) underwent a laparotomy, and catheters were inserted for sampling and infusion. A primed, constant, and continuous infusion of L-alanyl-L-[2-(15)N]glutamine (dipeptide groups) or L-[2-(15)N]glutamine (free L-glutamine groups), simultaneously with L-[ureido-(13)C,(2)H(2)]citrulline and L-[guanidino-(15)N(2),(2)H(2)]arginine, was given (steady-state model). Mice received the L-glutamine tracers intravenously (jugular vein) or enterally (duodenum). Enrichments of metabolites were measured by LC-MS. Arterial L-glutamine concentrations were the highest in the intravenous dipeptide group. L-Glutamine was converted to L-citrulline and L-arginine when L-[2-(15)N]glutamine and L-alanyl-L-[2-(15)N]glutamine were given by enteral or parenteral route. The contribution of L-glutamine to the de novo synthesis of L-citrulline and L-arginine was higher in the enteral groups when compared with the intravenous groups (P < 0.005). Therefore, the route of administration (enteral or parenteral) affects the contribution of L-glutamine, provided as free molecule or dipeptide, to the de novo synthesis of L-arginine in mice.
Petra G Boelens; Gerdien C Melis; Paul A van Leeuwen; Gabrie A ten Have; Nicolaas E Deutz
Related Documents :
12406198 - Maxadilan, the vasodilator/immunomodulator from lutzomyia longipalpis sand fly saliva, ...
23258818 - Evaluation of a textile-based wearable system for the electrocardiogram monitoring in c...
19297408 - Swimming exercise at weaning improves glycemic control and inhibits the onset of monoso...
11833608 - Prevention of auditory dysfunction in hypothyroid tshr mutant mice by thyroxin treatmen...
19230768 - Self-reported physical activity behaviour; exercise motivation and information among da...
10404498 - Physiological responses and perceptions of exertion in a step aerobics session.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-09
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  291     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-06     Completed Date:  2006-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E683-90     Citation Subset:  IM    
Department of Surgery, Vrije Universiteit. Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Arginine / biosynthesis*,  blood
Citrulline / blood,  metabolism*
Enteral Nutrition
Glutamine / administration & dosage*,  blood,  metabolism*
Models, Biological*
Nitrogen Isotopes
Parenteral Nutrition
Reg. No./Substance:
0/Nitrogen Isotopes; 372-75-8/Citrulline; 56-85-9/Glutamine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of aripiprazole on alcohol intake in an animal model of high-alcohol drinking.
Next Document:  Body mass index, free insulin-like growth factor I, and physical function among older adults: result...