| Round spermatids stained with mitotracker can be used to produce offspring more simply. | |
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MedLine Citation:
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PMID: 15984163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although both intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) are used in infertility treatments, the rate of offspring achieved with ROSI is low compared with that achieved with ICSI. The difficulty in correctly selecting round spermatids from testicular cells is one of the causes of this phenomenon. We easily selected live round spermatids from testicular cells stained with 20 nM MitoTracker, which visualizes mitochondria without killing the cell. Using this method, we divided round spermatids into three groups based on the polarization of their mitochondria, and performed ROSI. The rate of successful offspring achieved with MitoTracker-stained ROSI was the same in all groups. This indicates that changes in the polarization of mitochondria in round spermatids are not directly related to the developmental capacity of subsequently fertilized embryos. Because this staining has no harmful effects on embryo development, the selection of spermatids by MitoTracker under a fluorescence microscope should be useful in research into and the treatment of infertility. |
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Authors:
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Takafusa Hikichi; Satoshi Kishigami; Nguyen Van Thuan; Hiroshi Ohta; Eiji Mizutani; Sayaka Wakayama; Teruhiko Wakayama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zygote (Cambridge, England) Volume: 13 ISSN: 0967-1994 ISO Abbreviation: Zygote Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-06-29 Completed Date: 2005-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9309124 Medline TA: Zygote Country: England |
Other Details:
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Languages: eng Pagination: 55-61 Citation Subset: IM |
Affiliation:
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Center for Developmental Biology, RIKEN Kobe, Kobe, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes* Animals Cell Polarity Cell Shape Cytological Techniques* Embryonic Development Female Fertilization in Vitro / methods* Male Mice Mice, Inbred ICR Mice, Inbred Strains Mitochondria / physiology Pregnancy Spermatids / cytology* Testis / cytology |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/mitotracker green FM |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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