Document Detail

Round spermatids stained with mitotracker can be used to produce offspring more simply.
MedLine Citation:
PMID:  15984163     Owner:  NLM     Status:  MEDLINE    
Although both intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) are used in infertility treatments, the rate of offspring achieved with ROSI is low compared with that achieved with ICSI. The difficulty in correctly selecting round spermatids from testicular cells is one of the causes of this phenomenon. We easily selected live round spermatids from testicular cells stained with 20 nM MitoTracker, which visualizes mitochondria without killing the cell. Using this method, we divided round spermatids into three groups based on the polarization of their mitochondria, and performed ROSI. The rate of successful offspring achieved with MitoTracker-stained ROSI was the same in all groups. This indicates that changes in the polarization of mitochondria in round spermatids are not directly related to the developmental capacity of subsequently fertilized embryos. Because this staining has no harmful effects on embryo development, the selection of spermatids by MitoTracker under a fluorescence microscope should be useful in research into and the treatment of infertility.
Takafusa Hikichi; Satoshi Kishigami; Nguyen Van Thuan; Hiroshi Ohta; Eiji Mizutani; Sayaka Wakayama; Teruhiko Wakayama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  13     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-06-29     Completed Date:  2005-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  55-61     Citation Subset:  IM    
Center for Developmental Biology, RIKEN Kobe, Kobe, Japan.
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MeSH Terms
Cell Polarity
Cell Shape
Cytological Techniques*
Embryonic Development
Fertilization in Vitro / methods*
Mice, Inbred ICR
Mice, Inbred Strains
Mitochondria / physiology
Spermatids / cytology*
Testis / cytology
Reg. No./Substance:
0/Aldehydes; 0/mitotracker green FM

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