Document Detail


Rotational echo double resonance detection of cross-links formed in mussel byssus under high-flow stress.
MedLine Citation:
PMID:  10400649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
13C2H rotational echo double resonance NMR has been used to provide the first evidence for the formation of quinone-derived cross-links in mussel byssal plaques. Labeling of byssus was achieved by allowing mussels to filter feed from seawater containing L-[phenol-4-13C]tyrosine and L-[ring-d4]tyrosine for 2 days. Plaques and threads were harvested from two groups of mussels over a period of 28 days. One group was maintained in stationary water while the other was exposed to turbulent flow at 20 cm/s. The flow-stressed byssal plaques exhibited significantly enhanced levels of 5, 5'-di-dihydroxyphenylalanine cross-links. The average concentration of di-dihydroxyphenylalanine cross-links in byssal plaques is 1 per 1800 total protein amino acid residues.
Authors:
L M McDowell; L A Burzio; J H Waite; J Schaefer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-19     Completed Date:  1999-08-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  20293-5     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bivalvia / chemistry*
Cross-Linking Reagents / chemistry
Nuclear Magnetic Resonance, Biomolecular / methods*
Tyrosine / chemistry
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 55520-40-6/Tyrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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