| Rotation algorithm for determining cycle thresholds in Real-Time Polymerase Chain Reactions. | |
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MedLine Citation:
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PMID: 18002372 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Real Time-Polymerase Chain Reaction (RT-PCR) is a methodology that is widely used to amplify and quantify specific sections of nucleic acid strands. A key aspect of RT-PCR is to determine the reaction cycle that produces a positive PCR signal. This cycle is proportional to the relative concentration of nucleic acid and produces a linear curve when plotted vs. the log of the sample concentration. The cycle that produces a positive PCR signal is called the elbow or more precisely the Cycle Threshold (Ct) value. This paper describes a novel Rotation Algorithm that determines the Ct value and is less susceptible to signal interferences. |
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Authors:
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Ronald T Kurnik |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference Volume: 2007 ISSN: 1557-170X ISO Abbreviation: Conf Proc IEEE Eng Med Biol Soc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-11-16 Completed Date: 2008-04-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101243413 Medline TA: Conf Proc IEEE Eng Med Biol Soc Country: United States |
Other Details:
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Languages: eng Pagination: 1977-80 Citation Subset: IM |
Affiliation:
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Roche Molecular Systems, Pleasanton, CA 94588, USA. ronald.kurnik@roche.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Computational Biology / instrumentation, methods* Mathematics Models, Statistical Models, Theoretical Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction / instrumentation*, methods* Sequence Analysis, DNA / methods Signal Processing, Computer-Assisted* Spectrometry, Fluorescence Temperature Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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