Document Detail


Rosmarinic acid synthase is a new member of the superfamily of BAHD acyltransferases.
MedLine Citation:
PMID:  17047986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Purification of rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase) from suspension cells of Coleus blumei Benth. (Lamiaceae) by fractionated ammonium sulphate precipitation, hydrophobic interaction chromatography and two affinity chromatography steps led to the identification of peptide sequences, which enabled a PCR-based approach to isolate the full-length cDNA encoding this enzyme. The open reading frame of the cDNA had a length of 1290 base pairs encoding a protein of 430 amino acid residues with a molecular mass of 47,932 Da with typical characteristics of an acyltransferase of the BAHD superfamily. The cDNA was heterologously expressed in Escherichia coli. The enzyme displayed the activity of rosmarinic acid synthase using 4-coumaroyl- and caffeoyl-coenzyme A and 4-hydroxyphenyllactate as well as 3.4-dihydroxyphenyllactate as substrates. Shikimic acid and quinic acid were not able to serve as hydroxycinnamoyl acceptors. This therefore is the first report of the cDNA-cloning of a rosmarinic acid synthase.
Authors:
Anja Berger; Juliane Meinhard; Maike Petersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-19
Journal Detail:
Title:  Planta     Volume:  224     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-19     Completed Date:  2007-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1503-10     Citation Subset:  IM    
Affiliation:
Institut für Pharmazeutische Biologie, Philipps-Universität Marburg, Deutschhausstr. 17A, 35037, Marburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / chemistry,  genetics,  metabolism*
Amino Acid Sequence
Base Sequence
DNA, Complementary
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA, Complementary; EC 2.3.-/Acyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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