| Rosmarinic acid synthase is a new member of the superfamily of BAHD acyltransferases. | |
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MedLine Citation:
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PMID: 17047986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Purification of rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase) from suspension cells of Coleus blumei Benth. (Lamiaceae) by fractionated ammonium sulphate precipitation, hydrophobic interaction chromatography and two affinity chromatography steps led to the identification of peptide sequences, which enabled a PCR-based approach to isolate the full-length cDNA encoding this enzyme. The open reading frame of the cDNA had a length of 1290 base pairs encoding a protein of 430 amino acid residues with a molecular mass of 47,932 Da with typical characteristics of an acyltransferase of the BAHD superfamily. The cDNA was heterologously expressed in Escherichia coli. The enzyme displayed the activity of rosmarinic acid synthase using 4-coumaroyl- and caffeoyl-coenzyme A and 4-hydroxyphenyllactate as well as 3.4-dihydroxyphenyllactate as substrates. Shikimic acid and quinic acid were not able to serve as hydroxycinnamoyl acceptors. This therefore is the first report of the cDNA-cloning of a rosmarinic acid synthase. |
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Authors:
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Anja Berger; Juliane Meinhard; Maike Petersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-19 |
Journal Detail:
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Title: Planta Volume: 224 ISSN: 0032-0935 ISO Abbreviation: Planta Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-19 Completed Date: 2007-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 1503-10 Citation Subset: IM |
Affiliation:
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Institut für Pharmazeutische Biologie, Philipps-Universität Marburg, Deutschhausstr. 17A, 35037, Marburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyltransferases
/
chemistry,
genetics,
metabolism* Amino Acid Sequence Base Sequence DNA, Complementary Molecular Sequence Data Phylogeny Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; EC 2.3.-/Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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