Document Detail


Rose bengal acetate photodynamic therapy-induced autophagy.
MedLine Citation:
PMID:  20935508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy (PDT), an anticancer therapy requiring the exposure of cells or tissue to a photosensitizing drug followed by irradiation with visible light of the appropriate wavelength, induces cell death by the efficient induction of apoptotic as well as non-apoptotic mechanisms, such as necrosis and autophagy, or a combination of all three mechanisms. However, the exact role of autophagy in photodynamic therapy is still a matter of debate. To understand the role of autophagy in PDT, we investigated the induction of autophagy in HeLa cells photosensitized with Rose Bengal Acetate (RBAc). After incubation with Rose Bengal Acetate (10-5 M), HeLa cells were irradiated for 90 seconds (green LED DPL 305, emitting at 530 +15 nm to obtain 1.6 J/cm2 as the total light dose) and allowed to recover for 72 h. Induction of autophagy and apoptosis were observed with peaks at 8 h and 12 h after irradiation, respectively. Autophagy was detected by biochemical (Western Blotting for the LC3B protein) and morphological criteria (TEM, cytochemistry). In addition, the pan-caspase inhibitor, z-VAD, was unable to completely prevent cell death. The simultaneous onset of apoptosis and autophagy following Rose Bengal Acetate PDT is of remarkable interest in light of the findings that autophagy can result in the class II presentation of antigens and thus, explain why low dose PDT can yield anti-tumor immune responses.
Authors:
Luciana Dini; Valentina Inguscio; Bernardetta Tenuzzo; Elisa Panzarini
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Publication Detail:
Type:  Journal Article     Date:  2010-11-15
Journal Detail:
Title:  Cancer biology & therapy     Volume:  10     ISSN:  1555-8576     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2011-01-05     Completed Date:  2011-03-10     Revised Date:  2013-02-05    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1048-55     Citation Subset:  IM    
Affiliation:
Dept. of Biological and Environmental Science and Technology, University of Salento, Italy. luciana.dini@unisalento.it
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*,  radiation effects
Autophagy*
Cell Survival / drug effects,  radiation effects
Endoplasmic Reticulum / drug effects,  radiation effects
HeLa Cells
Humans
Immunoblotting
Light*
Lysosomes / drug effects,  radiation effects
Mitochondria / drug effects,  radiation effects
Photochemotherapy*
Rose Bengal / analogs & derivatives*,  therapeutic use
Chemical
Reg. No./Substance:
0/Rose Bengal acetate; 11121-48-5/Rose Bengal

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