| Root canal microbiota of dogs' teeth with periapical lesions induced by two different methods. | |
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MedLine Citation:
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PMID: 16997126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The microbial composition was investigated in root canals of dogs' teeth with periapical lesions induced by 2 different methods: open versus sealed canals. STUDY DESIGN: Teeth from Group I (n = 16) were left open for a week, then sealed with composite resin for 120 days. The teeth from Group II (n = 16) were left open for the same period. Microbiological samples from the root canals were collected and processed by the anaerobic technique for identification and counting of microorganisms after establishment of periapical reactions. RESULTS: Seventy-four cultivable isolates were recovered in sealed canals (Group I). Strict anaerobes accounted for 64.9% of all species isolated, and gram-negative microorganisms accounted for 55.4%. Microbial genera most frequently isolated were Prevotella, Fusobacterium, Peptostreptococcus, Streptococcus, Enterococcus, Clostridium, and Porphyromonas. Statistical analysis by Pearson chi-square or Fisher's test revealed positive association between sealed teeth and strict anaerobes (P < .05). In open canals (Group II), from a total of 58 cultivable isolates, 19% were strict anaerobes and 81% facultative anaerobes, with predominance of gram-positive species (75.8%). Genera most frequently isolated were Streptococcus, Propionibacterium, Staphylococcus, Neisseria, and Prevotella. CONCLUSION: Strict anaerobes were most frequently found in sealed teeth rather than in the teeth with canals left exposed to the oral cavity for 4 months. Therefore, the method that induced periapical inflammatory lesions by intentional oral exposure, followed by tooth sealing, produced root canal microbiota similar to the same found in humans. |
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Authors:
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Flaviana Bombarda de Andrade Ferreira; Helena Rosa Campos Rabang; Ericka Tavares Pinheiro; Cícero Romão Gadê-Neto; Alexandre Augusto Zaia; Caio Cezar Randi Ferraz; Francisco José de Souza-Filho; Brenda Paula Figueiredo de Almeida Gomes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-10 |
Journal Detail:
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Title: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics Volume: 102 ISSN: 1528-395X ISO Abbreviation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-25 Completed Date: 2006-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9508562 Medline TA: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Country: United States |
Other Details:
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Languages: eng Pagination: 564-70 Citation Subset: D; IM |
Affiliation:
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Department of Restorative Dentistry, Endodontic Area, Bauru Dental School, University of São Paulo, São Paulo, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteria, Anaerobic / growth & development Bacteriological Techniques Colony Count, Microbial Dental Pulp Cavity / microbiology* Dogs Female Gram-Negative Bacteria / growth & development Gram-Negative Facultatively Anaerobic Rods / growth & development Gram-Positive Bacteria / growth & development Models, Animal Periapical Periodontitis / microbiology* |
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