Document Detail


Rolled-up transparent microtubes as two-dimensionally confined culture scaffolds of individual yeast cells.
MedLine Citation:
PMID:  19107283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transparent oxide rolled-up microtube arrays were constructed on Si substrates by the deposition of a pre-stressed oxide layer on a patterned photoresist sacrificial layer and the subsequent removal of this sacrificial layer. These microtubes as well as their arrays can be well positioned onto a chip for further applications, while their dimensions (e.g. length, diameter and wall thickness) are controlled by tunable parameters of the fabrication process. Due to the unique tubular structure and optical transparency, such rolled-up microtubes can serve as well-defined two-dimensionally (2D) confined cell culture scaffolds. In our experiments, yeast cells exhibit different growth behaviors (i.e. their arrangement) in microtubes with varied diameters. In an extremely small microtube the yeast cell becomes highly elongated during growth but still survives. Detailed investigations on the behavior of individual yeast cells in a single microtube are carried out in situ to elucidate the mechanical interaction between microtubes and the 2D confined cells. The confinement of tubular channels causes the rotation of cell pairs, which is more pronounced in smaller microtubes, leading to different cellular assemblies. Our work demonstrates good capability of rolled-up microtubes for manipulating individual and definite cells, which promises high potential in lab-on-a-chip applications, for example as a bio-analytic system for individual cells if integrated with sensor functionalities.
Authors:
Gaoshan Huang; Yongfeng Mei; Dominic J Thurmer; Emica Coric; Oliver G Schmidt
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Publication Detail:
Type:  Journal Article     Date:  2008-10-23
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-03-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  263-8     Citation Subset:  IM    
Affiliation:
Institute for Integrative Nanosciences, IFW Dresden, Helmholtzstr. 20, D-01069, Dresden, Germany. g.huang@ifw-dresden.de
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques*
Saccharomyces cerevisiae / cytology*
Tissue Scaffolds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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