| Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis. | |
| | |
MedLine Citation:
|
PMID: 22669117 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Aim:To investigate the inhibitory effects of heparin on PC-3M cells proliferation in vitro and B16-F10-luc-G5 cells metastasis in Balb/c nude mice and identify the protein expression patterns to elucidate the action mechanism of heparin.Methods:Human prostate cancer PC-3M cells were incubated with heparin 0.5 to 125 μg/mL for 24 h. The proliferation of PC-3M cells was assessed by MTS assay. BrdU incoporation and Ki67 expression were detected using a high content screening (HCS) assay. The cell cycle and apoptosis of PC-3M cells were tested by flow cytometry. B16-F10-luc-G5 cardinoma cells were injected into the lateral tail vein of 6-week old male Balb/c nude mice and heparin 30 mg/kg was administered iv 30 min before and 24 h after injection. The metasis of B16-F10-luc-G5 cells was detected by bioluminescence assay. Activated partial thromboplastin time (APTT) and hemorheological parameters were measured on d 14 after injection of B16-F10-luc-G5 carcinoma cells in Balb/c mice. The global protein changes in PC-3M cells and frozen lung tissues from mice burdened with B16-F10-luc-G5 cells were determined by 2-dimensional gel electrophoresis and image analysis. The protein expression of vimentin and 14-3-3 zeta/delta was measured by Western blot. The mRNA transcription of vimentin, transforming growth factor (TGF)-β, E-cadherin, and α(v)-integrin was measured by RT-PCR.Results:Heparin 25 and 125 μg/mL significantly inhibited the proliferation, arrested the cells in G(1) phase, and suppressed BrdU incorporation and Ki67 expression in PC-3M cells compared with the model group. But it had no significant effect on apoptosis of PC-3M cells. Heparin 30 mg/kg markedly inhibits the metastasis of B16-F10-luc-G5 cells on day 8. Additionally, heparin administration maintained relatively normal red blood hematocrit but had no influence on APTT in nude mice burdened with B16-F10-luc-G5 cells. Thirty of down-regulated protein spots were identified after heparin treatment, many of which are related to tumor development, extracellular signaling, energy metabolism, and cellular proliferation. Vimentin and 14-3-3 zeta/delta were identified in common in PC-3M cells and the lungs of mice bearing B16-F10-luc-G5 carcinoma cells. Heparin 25 and 125 μg/mL decreased the protein expression of vimentin and 14-3-3 zeta/delta and the mRNA expression of α(v)-integrin. Heparin 125 μg/mL decreased vimentin and E-cadherin mRNA transcription while increased TGF-β mRNA transcription in the PC-3M cells, but the differences were not significant. Transfection of vimentin-targeted siRNA for 48 h significantly decreased the BrdU incoporation and Ki67 expression in PC-3M cells.Conclusion:Heparin inhibited PC-3M cell proliferation in vitro and B16-F10-luc-G5 cells metastasis in nude mice by inhibition of vimentin, 14-3-3 zeta/delta, and α(v)-integrin expression. |
| | |
Authors:
|
Yan Pan; Li-Jun Zhong; Hong Zhou; Xin Wang; Kui Chen; Hao-Peng Yang; Yilixiati Xiaokaiti; Aikebaier Maimaiti; Ling Jiang; Xue-Jun Li |
Related Documents
:
|
2415247 - Growth and differentiation of human esophageal carcinoma cell lines. 20665227 - A recombinant peroxisome proliferator response element-driven luciferase assay for eval... 2827967 - Monoclonal antibodies against human esophageal carcinoma cell lines. 1710547 - Constitutive il-2 expression in htlv-i-infected leukaemic t cell lines. 9199687 - A case of giant cell reparative granuloma of the petrous bone: demonstration of the pro... 22037307 - Control of drosophila endocycles by e2f and crl4(cdt2). |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Acta pharmacologica Sinica Volume: 33 ISSN: 1745-7254 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2012 Jun |
Date Detail:
|
Created Date: 2012-06-06 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: United States |
Other Details:
|
Languages: eng Pagination: 798-808 Citation Subset: IM |
Affiliation:
|
State Key Laboratory of Natural and Biomimetic Drugs, Department of Pharmacology, School of Basic Medical Sciences, Peking University and Institute of System Biomedicine, Peking University, Beijing 100191, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: [7]-Helicene: a chiral molecular tweezer for silver(i) salts.
Next Document: Population pharmacokinetics modeling of levetiracetam in Chinese children with epilepsy.