Document Detail

Roles of polysialic acid in migration and differentiation of neural stem cells.
MedLine Citation:
PMID:  20816158     Owner:  NLM     Status:  MEDLINE    
Polysialic acid, a homopolymer of alpha2,8-linked sialic acid, is one of the carbohydrates expressed on neural precursors in the embryonic and adult brain. Polysialic acid, synthesized by two polysialyltransferases (ST8SiaII and ST8SiaIV), mainly modulates functions of the neural cell adhesion molecule (NCAM). Polysialic acid-deficient mice demonstrated that polysialylated NCAM plays crucial roles in various steps of neural development, such as cell survival and cell migration of neural precursors, neuronal guidance, and synapse formation. However, the mechanisms of the diverse phenotypes and molecules affected by polysialic acid remain to be defined. To study the roles of polysialic acid on neural stem cells, analyses of neural stem cells from polysialic acid-deficient and NCAM-deficient mice are useful. Here, we describe how to prepare neural precursor cells from mouse brain and how to analyze migration and differentiation of neurosphere cells in vitro.
Kiyohiko Angata; Minoru Fukuda
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in enzymology     Volume:  479     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-09-06     Completed Date:  2010-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-36     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Tumor Microenvironment Program, Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
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MeSH Terms
Cell Differentiation* / drug effects
Cell Movement / drug effects,  physiology
Cell Survival
Cells, Cultured
Genetic Vectors
Lentivirus / genetics
Mice, Knockout
Neural Stem Cells / cytology*,  drug effects
Sialic Acids / genetics,  metabolism*,  pharmacology
Grant Support
Reg. No./Substance:
0/Sialic Acids; 0/polysialic acid

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