| Roles of polysialic acid in migration and differentiation of neural stem cells. | |
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MedLine Citation:
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PMID: 20816158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polysialic acid, a homopolymer of alpha2,8-linked sialic acid, is one of the carbohydrates expressed on neural precursors in the embryonic and adult brain. Polysialic acid, synthesized by two polysialyltransferases (ST8SiaII and ST8SiaIV), mainly modulates functions of the neural cell adhesion molecule (NCAM). Polysialic acid-deficient mice demonstrated that polysialylated NCAM plays crucial roles in various steps of neural development, such as cell survival and cell migration of neural precursors, neuronal guidance, and synapse formation. However, the mechanisms of the diverse phenotypes and molecules affected by polysialic acid remain to be defined. To study the roles of polysialic acid on neural stem cells, analyses of neural stem cells from polysialic acid-deficient and NCAM-deficient mice are useful. Here, we describe how to prepare neural precursor cells from mouse brain and how to analyze migration and differentiation of neurosphere cells in vitro. |
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Authors:
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Kiyohiko Angata; Minoru Fukuda |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in enzymology Volume: 479 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-12-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 25-36 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Tumor Microenvironment Program, Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation* / drug effects Cell Movement / drug effects, physiology Cell Survival Cells, Cultured Female Genetic Vectors Lentivirus / genetics Male Mice Mice, Knockout Neural Stem Cells / cytology*, drug effects Sialic Acids / genetics, metabolism*, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA33895/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sialic Acids; 0/polysialic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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