Document Detail

Roles of p38 and ERK MAP kinases in IL-8 expression in TNF-alpha- and dexamethasone-stimulated human periodontal ligament cells.
MedLine Citation:
PMID:  16949835     Owner:  NLM     Status:  MEDLINE    
Orthodontic tooth movement is recognized as a pro-inflammatory stressor of human periodontal ligament (hPDL) cells. However, the cell-signaling pathways linking interleukin-8 (IL-8), intercellular adhesion molecule-1 (ICAM-1), pro-inflammatory cytokines, and dexamethasone in hPDL cells have not been well elucidated. In this study, we investigated the role of mitogen-activated protein (MAP) kinases in dexamethasone- and TNF-alpha-induced IL-8 and ICAM-1 expression in hPDL cells. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAP kinase activation and IkappaB degradation were determined by Western blot analysis, and ICAM-1 expression was determined by RT-PCR and FACS analysis. TNF-alpha increased IL-8 mRNA expression and protein secretion in a dose- and time-dependent manner. Dexamethasone suppressed TNF-alpha-induced IL-8 production in a dose-dependent manner. In addition, dexamethasone inhibited TNF-alpha-induced phosphorylation of p38 MAP kinase and extracellular-regulated kinases (ERKs), IkappaB degradation, and NF-kappaB activation. Selective inhibitors for ERKs and p38 attenuated TNF-alpha-induced IL-8 and ICAM-1 expression in the presence and absence of dexamethasone, indicating that MAP kinases play a role in the response of hDPL cells to TNF-alpha. Furthermore, these results suggest that inflammatory cytokine- and dexamethasone-induced IL-8 and ICAM-1, produced via a MAP kinase pathway, may serve as an important mediator of PDL immunoregulation involved in bone remodeling during orthodontic tooth movement.
Hwa-Jeong Lee; Jin-Woo Cho; Sang-Cheol Kim; Kyung-Hwa Kang; Sun-Kyung Lee; Sung-Hee Pi; Suk-Keun Lee; Eun-Cheol Kim
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Publication Detail:
Type:  Journal Article     Date:  2006-09-01
Journal Detail:
Title:  Cytokine     Volume:  35     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-10-09     Completed Date:  2006-12-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
Department of Oral and Maxillofacial Pathology, College of Dentistry, Wonkwang University, Iksan, Republic of Korea.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology
Bone Remodeling / physiology
Cells, Cultured
Dexamethasone / pharmacology*
Extracellular Signal-Regulated MAP Kinases / physiology*
Intercellular Adhesion Molecule-1 / biosynthesis,  genetics
Interleukin-8 / biosynthesis*,  genetics*
Orthodontics, Corrective
Periodontal Ligament / cytology,  drug effects,  enzymology*
Signal Transduction / physiology
Tooth Migration / metabolism
Tumor Necrosis Factor-alpha / physiology*
p38 Mitogen-Activated Protein Kinases / physiology*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Interleukin-8; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1; 50-02-2/Dexamethasone; EC Signal-Regulated MAP Kinases; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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