| Roles of major oligosaccharides on Cry j 1 in human immunoglobulin E and T cell responses. | |
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MedLine Citation:
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PMID: 15144470 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have demonstrated that carbohydrates in Cry j 1, the major allergen of Cryptomeria japonica pollen, play a major role in promoting Cry j 1-specific Th2 response. However, little is known as to whether the carbohydrates directly participate in allergic responses. OBJECTIVE: We sought to determine whether Cry j 1-related oligosaccharides function as IgE and/or T cell epitopes. In addition, the regulatory effect of Cry j 1-related oligosaccharide on Cry j 1-specific T cell responses was investigated. METHODS: Two monovalent oligosaccharides largely found on Cry j 1, Manalpha1-6(Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc (M3FX), and GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)(Xylbeta1-2)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc (GN2M3FX) were prepared. Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc (M9A) was used as control. Competitive inhibition ELISA for Cry j 1-specific IgE was performed using these oligosaccharides as inhibitors. In addition, T cell lines specific for Cry j 1 or purified protein derivative of Mycobacterium tubecurosis (PPD) were established, and cellular responses against these oligosaccharides were investigated in the presence or absence of the respective antigens. RESULTS: Overall, neither M3FX nor GN2M3FX displayed inhibitory effect on the binding between IgE and Cry j 1. In addition, M3FX did not by itself stimulate Cry j 1 or PPD-specific T cells. However, M3FX significantly inhibited Cry j 1-induced proliferation and IL-4 production in Cry j 1-specific T cells. Such an inhibitory effect was not seen in PPD-specific T cell responses. CONCLUSION: These results suggest that Cry j 1-related oligosaccharides are not major epitopes for IgE or T cells. However, these oligosaccharides have a novel potential to inhibit Cry j 1-specific T cell responses selectively. |
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Authors:
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M Okano; Y Kimura; K Kino; Y Michigami; S Sakamoto; Y Sugata; M Maeda; F Matsuda; M Kimura; T Ogawa; K Nishizaki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 34 ISSN: 0954-7894 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-05-17 Completed Date: 2004-08-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
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Languages: eng Pagination: 770-8 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. mokano@cc.okayama-u.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Allergens / chemistry, immunology* Cell Line Child Cryptomeria / immunology* Enzyme-Linked Immunosorbent Assay / methods Female Humans Immunoglobulin E / analysis* Interferon-gamma / analysis Interleukin-4 / analysis Lymphocyte Activation Male Middle Aged Oligosaccharides / analysis, immunology* Plant Proteins / chemistry, immunology* Pollen Rhinitis, Allergic, Seasonal / immunology* Th2 Cells / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Cry j I protein, Cryptomeria japonica; 0/Oligosaccharides; 0/Plant Proteins; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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