| Roles of gene transcription and PKA subtype activation in maturation of murine oocytes. | |
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MedLine Citation:
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PMID: 12052234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aims of this study were to examine the role of transcription and the coincident involvement of type I and type II protein kinase A (PKA) in the resumption of meiosis in murine cumulus-oocyte complexes (COCs) using the transcriptional inhibitors 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin. The first series of experiments was designed to: (i) characterize the role of transcription in gonadotrophin-mediated and spontaneous maturation of murine oocytes; (ii) examine the roles of specific gonadotrophins (FSH versus hCG) and cumulus cells in transcriptionally mediated oocyte maturation; and (iii) determine the reversibility of the transcriptional arrest of meiosis. In the presence of FSH, transcriptional inhibitors arrested germinal vesicle breakdown (GVBD) (DRB: 2 +/- 2% and control: 76 +/- 2%; alpha-amanitin: 4 +/- 4% and control: 70 +/- 4%). Furthermore, cumulus cells were required for transcriptional inhibitors to arrest GVBD (DRB with cumulus cells: 0 +/- 15%; DRB without cumulus cells: 94 +/- 13%; alpha-amanitin with cumulus cells: 15 +/- 2%; alpha-amanitin without cumulus cells: 99 +/- 2%). Thus, in mice, FSH-mediated GVBD uses a transcriptional mechanism, which probably occurs within the cumulus cell compartment. In a second series of experiments, the role of transcription in mediating the resumption of meiosis after activation of either type I or type II PKA was examined. Activation of type I PKA in murine COCs resulted in an arrest of GVBD that was independent of a transcriptional event (with DRB: 7 +/- 9% GVBD; without DRB: 11 +/- 9% GVBD). In contrast, activation of type II PKA resulted in a resumption of meiosis, which required the occurrence of gene transcription (with DRB: 12 +/- 9% GVBD; without DRB: 80 +/- 9% GVBD). As FSH binding to cumulus cells activates the PKA second messenger system, our results indicate that, in cultured murine COCs, FSH binding to cumulus cells results in the activation of type II PKA, which, in turn, mediates a downstream transcriptional event required for the initiation of GVBD. |
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Authors:
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K F Rodriguez; R M Petters; A E Crosier; C E Farin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: 123 ISSN: 1470-1626 ISO Abbreviation: Reproduction Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-07 Completed Date: 2002-07-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
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Languages: eng Pagination: 799-806 Citation Subset: IM |
Affiliation:
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Department of Animal Science, North Carolina State University, 231B Polk Hall, Raleigh, NC 27695-7621, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amanitins
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pharmacology Animals Cells, Cultured Chorionic Gonadotropin / pharmacology Cyclic AMP-Dependent Protein Kinase Type II Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors, metabolism* Dichlororibofuranosylbenzimidazole / pharmacology Enzyme Activation / physiology* Enzyme Inhibitors / pharmacology Female Follicle Stimulating Hormone / pharmacology Isoenzymes / metabolism Mice Mice, Inbred Strains Oogenesis / physiology* Transcription, Genetic / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Amanitins; 0/Chorionic Gonadotropin; 0/Enzyme Inhibitors; 0/Isoenzymes; 53-85-0/Dichlororibofuranosylbenzimidazole; 9002-68-0/Follicle Stimulating Hormone; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinase Type II; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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