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Roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells with rat pancreatic cells in the treatment of rats with diabetes mellitus.
MedLine Citation:
PMID:  25289028     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of the present study was to investigate the roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) with rat pancreatic cells in the treatment of rats with diabetes mellitus. hUC-MSCs were isolated and passaged, followed by Transwell co-culture with rat pancreatic cells. The induced islet-like cell clusters were transplanted into the renal capsule in rats with streptozotocin (STZ)-induced diabetes mellitus. The effects of co-culture on blood glucose levels in rats were observed. The isolated hUC-MSCs expressed the specific surface markers, including cluster of differentiation 44 (CD44) (91.4%), CD29 (91.3%) and CD105 (99.2%). Following co-culture with hUC-MSCs for 7 and 10 days, the rat pancreatic cells were strongly stained by pancreatic and duodenal homeobox-1 and human insulin. The insulin and C-peptide concentrations were increased significantly compared to the pure culture group. One week following the transplantation of induced islet-like cells into the renal capsule, the blood glucose level of rats in the STZ experimental group was significantly lower than that of the STZ control group. There were notable 5-bromo-2'-deoxyuridine-positive nuclei and insulin-positive cytoplasm in the renal capsule following cell transplantation. Therefore, co-culture of hUC-MSCs with rat pancreatic cells can lower the blood glucose levels in rats with diabetes mellitus.
Authors:
Guangyu Wang; Yong Li; Yu Wang; Yu Dong; Fu-Sheng Wang; Yi Ding; Yudong Kang; Xuying Xu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-22
Journal Detail:
Title:  Experimental and therapeutic medicine     Volume:  8     ISSN:  1792-0981     ISO Abbreviation:  Exp Ther Med     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-10-7     Completed Date:  -     Revised Date:  2014-10-8    
Medline Journal Info:
Nlm Unique ID:  101531947     Medline TA:  Exp Ther Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1389-1396     Citation Subset:  -    
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