Document Detail

Roles of Necl-5/poliovirus receptor and Rho-associated kinase (ROCK) in the regulation of transformation of integrin alpha(V)beta(3)-based focal complexes into focal adhesions.
MedLine Citation:
PMID:  18364349     Owner:  NLM     Status:  MEDLINE    
Focal complexes are continuously formed and transformed into focal adhesions during cell movement. We previously demonstrated that Necl-5 co-localizes with integrin alpha(V)beta(3) at focal complexes, whereas Necl-5 does not localize at focal adhesions in moving NIH3T3 cells, suggesting that Necl-5 may be dissociated from integrin alpha(V)beta(3) during the transformation of focal complexes into focal adhesions, but the underlying mechanism remains unknown. Here, we explore the roles of Necl-5 and Rho-associated kinase (ROCK) in the regulation of the transformation of focal complexes into focal adhesions. We found that inhibition of Necl-5 expression and expression of a constitutively active mutant of ROCK1 enhanced, whereas treatment with a ROCK inhibitor Y-27632 inhibited the transformation of focal complexes into focal adhesions. In HEK293 cells ectopically expressing Necl-5 and integrin alpha(V)beta(3), treatment of cells with Y-27632 increased the binding of Necl-5 to clustered integrin alpha(V)beta(3). The experiments using inhibitors of myosin ATPase and actin polymerization revealed that actomyosin-driven contractility exerts a similar function as ROCK. The phosphorylation of integrin beta(3) at Tyr(747), which is known to be critical for the formation of focal adhesions, plays a pivotal role for the interaction between Necl-5 and integrin alpha(V)beta(3). These results indicate that the transformation of focal complexes into focal adhesions is negatively and positively regulated by Necl-5 and ROCK, respectively, and that ROCK-dependent actomyosin-driven contractility is a critical determinant for the regulation of the interaction between Necl-5 and integrin alpha(V)beta(3).
Yuichi Nagamatsu; Yoshiyuki Rikitake; Motonori Takahashi; Yuko Deki; Wataru Ikeda; Ken-ichi Hirata; Yoshimi Takai
Related Documents :
11496289 - Overexpression of the focal adhesion kinase (p125fak) in the vascular smooth muscle cel...
21832119 - Estrogen receptor-β and fetoplacental endothelial prostanoid biosynthesis: a link to cl...
15923189 - Git1 is a scaffold for erk1/2 activation in focal adhesions.
16489009 - Minimal active domain and mechanism of action of the angiogenesis inhibitor histidine-r...
15729589 - Entamoeba histolytica: focal adhesion kinase and erk phosphorylation are altered in the...
18445079 - Altered adhesive structures and their relation to rhogtpase activation in merlin-defici...
22391569 - Microrna-125b inhibits tube formation of blood vessels through translational suppressio...
7768999 - Sequence and tissue distribution of the human integrin alpha 8 subunit: a beta 1-associ...
10069529 - Selective stimulation of l-arginine uptake contributes to shear stress-induced formatio...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-25
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  283     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14532-41     Citation Subset:  IM    
Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Focal Adhesions / metabolism*
Integrin alphaVbeta3 / genetics,  metabolism*
Phosphotyrosine / metabolism
Protein Binding
Protein Kinase Inhibitors / pharmacology
Receptors, Virus / genetics,  metabolism*
Transgenes / genetics
rho-Associated Kinases / antagonists & inhibitors,  metabolism*
Reg. No./Substance:
0/Integrin alphaVbeta3; 0/Protein Kinase Inhibitors; 0/Receptors, Virus; 0/poliovirus receptor; 21820-51-9/Phosphotyrosine; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  New insights into the alternative D-glucarate degradation pathway.
Next Document:  Peroxisomes as novel players in cell calcium homeostasis.