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Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype.
MedLine Citation:
PMID:  25270389     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Human mesenchymal stem cells (MSCs) are reported to have the capability of differentiating towards nucleus pulposus (NP)-like phenotype under specific culture conditions. So far, the effects of fibroblast growth factor (FGF)-2 and the cocktail effects of transforming growth factor (TGF)-beta and FGF-2 on MSCs remain unclear. Therefore, we designed this study to clarify these effects. MSCs were cultured in conditioned medium containing FGF-2 or TGF-beta/FGF-2, and compared with basal or TGF-beta medium. The groups with FGF-2 showed the increase of cell proliferation. Functional gene markers and novel NP markers decreased in FGF-2 group, together with functional protein expression. Pho-ERK1/2 and pho-Smad3 differed significantly in the two conditioned groups. All these results suggest FGF-2 promotes MSCs' proliferation, synergistically with TGF-beta. However, FGF-2 plays a negative role in cartilage homeostasis. We also demonstrate that FGF-2 has no positive effect in differentiating MSCs into NP-like cells, but hinders the acceleration effect of TGF-beta.
Authors:
Xiaopeng Zhou; Yiqing Tao; Jin Wang; Chengzhen Liang; Jun Wang; Hao Li; Qixin Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-1
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  -     ISSN:  1029-2292     ISO Abbreviation:  Growth Factors     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-1     Completed Date:  -     Revised Date:  2014-10-2    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-8     Citation Subset:  -    
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