| Role of zinc along with ascorbic acid and folic acid during long-term in vitro albumin glycation. | |
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MedLine Citation:
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PMID: 19849871 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study aimed to investigate the role of Zn alone and in the presence of ascorbic acid (AA) and folic acid (FA) in albumin glycation. Glycation was performed by incubations of bovine serum albumin with glucose at 37 degrees C along with Zn, AA or FA separately and Zn + AA or Zn + FA for 150 d. Glycation-mediated modifications were monitored as fluorescence of advanced glycation endproducts, carbonyl formation, beta aggregation (thioflavin T and Congo red dyes), albumin-bound Zn, thiol groups and glycated aggregate's toxicity in HepG2 cells. Zn inhibited glycation and beta aggregation, probably due to observed higher protein-bound Zn. It also protected protein thiols and increased cell survival. AA and FA enhanced glycation, which was lowered in Zn-co-incubated samples. FA increased albumin-bound Zn and showed maximum cell survival. Although these results warrant further in vivo investigation, the present data help in the understanding of the interplay of Zn with micronutrients in albumin glycation. |
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Authors:
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Rashmi Santosh Tupe; Vaishali Vilas Agte |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-23 |
Journal Detail:
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Title: The British journal of nutrition Volume: 103 ISSN: 1475-2662 ISO Abbreviation: Br. J. Nutr. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-26 Completed Date: 2010-02-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372547 Medline TA: Br J Nutr Country: England |
Other Details:
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Languages: eng Pagination: 370-7 Citation Subset: IM |
Affiliation:
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Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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pharmacology* Cell Survival / drug effects Congo Red / metabolism Folic Acid / pharmacology* Glycosylation / drug effects Glycosylation End Products, Advanced / metabolism Hep G2 Cells / drug effects, metabolism Humans Kinetics Protein Binding Serum Albumin, Bovine / drug effects, metabolism*, toxicity Sulfhydryl Compounds / analysis Thiazoles / analysis Zinc / metabolism, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Glycosylation End Products, Advanced; 0/Serum Albumin, Bovine; 0/Sulfhydryl Compounds; 0/Thiazoles; 2390-54-7/thioflavin T; 50-81-7/Ascorbic Acid; 573-58-0/Congo Red; 59-30-3/Folic Acid; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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