Document Detail


Role of zinc along with ascorbic acid and folic acid during long-term in vitro albumin glycation.
MedLine Citation:
PMID:  19849871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study aimed to investigate the role of Zn alone and in the presence of ascorbic acid (AA) and folic acid (FA) in albumin glycation. Glycation was performed by incubations of bovine serum albumin with glucose at 37 degrees C along with Zn, AA or FA separately and Zn + AA or Zn + FA for 150 d. Glycation-mediated modifications were monitored as fluorescence of advanced glycation endproducts, carbonyl formation, beta aggregation (thioflavin T and Congo red dyes), albumin-bound Zn, thiol groups and glycated aggregate's toxicity in HepG2 cells. Zn inhibited glycation and beta aggregation, probably due to observed higher protein-bound Zn. It also protected protein thiols and increased cell survival. AA and FA enhanced glycation, which was lowered in Zn-co-incubated samples. FA increased albumin-bound Zn and showed maximum cell survival. Although these results warrant further in vivo investigation, the present data help in the understanding of the interplay of Zn with micronutrients in albumin glycation.
Authors:
Rashmi Santosh Tupe; Vaishali Vilas Agte
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-23
Journal Detail:
Title:  The British journal of nutrition     Volume:  103     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  370-7     Citation Subset:  IM    
Affiliation:
Agharkar Research Institute, G. G. Agarkar Road, Pune 411004, India.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / pharmacology*
Cell Survival / drug effects
Congo Red / metabolism
Folic Acid / pharmacology*
Glycosylation / drug effects
Glycosylation End Products, Advanced / metabolism
Hep G2 Cells / drug effects,  metabolism
Humans
Kinetics
Protein Binding
Serum Albumin, Bovine / drug effects,  metabolism*,  toxicity
Sulfhydryl Compounds / analysis
Thiazoles / analysis
Zinc / metabolism,  pharmacology*
Chemical
Reg. No./Substance:
0/Glycosylation End Products, Advanced; 0/Serum Albumin, Bovine; 0/Sulfhydryl Compounds; 0/Thiazoles; 2390-54-7/thioflavin T; 50-81-7/Ascorbic Acid; 573-58-0/Congo Red; 59-30-3/Folic Acid; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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