| Role of xanthine oxidase and xanthine dehydrogenase systems in thymocyte apoptosis, induced by papaverine | |
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MedLine Citation:
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PMID: 11200484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been established that papaverine as well as other xenobiotics (dexamethasone and nitrosodimethylamine) [figure: see text] provoked the thymocyte death like apoptosis. The increase of the quantity of double-strand, single-strand DNA breaks and low molecular weight fragments of DNA preceded cell death. In papaverine-induced process of thymocyte apoptosis the total activity of xanthine oxidase in thymocytes strongly elevated long before their death, the conversion of xanthine dehydrogenase (D-form) to xanthinoxidase (O-form) and accumulation of O-form in the cultural medium took place. Direct stimulating effect of papaverine on O-form of enzyme in thymocyte lysate was revealed. The used digitonin thymocytes were divided into cytoplasmic and structural component fractions. It was shown that about 80% of total xanthinoxidase activity was concentrated in cytoplasma while only 20% of its activity was found in structural components. More higher ratio of xanthinoxidase/xanthindehydrogenase (XO/XDH) was observed and papaverine-induced changes of these enzyme forms activities were expressed more brightly in the structural components, than in the thymocyte cytoplasma. During the process of developing thymocytes apoptosis caused by papaverine the reaction of lipid peroxidation was intensified. XO-hypoxanthin system displaying prooxidant influence on cells increased the cytotoxic effect of papaverine but the presence of allopurinol or catalase and superoxidedismutase decreased it. Besides, cytotoxic action on thymocytes of allopurinol itself as well as hypoxanthin itself was revealed. |
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Authors:
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T O Kishko; N P Dmitrenko |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Ukrainskiĭ biokhimicheskiĭ zhurnal Volume: 72 ISSN: 0201-8470 ISO Abbreviation: Ukr. Biokhim. Zh. Publication Date: 2000 May-Jun |
Date Detail:
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Created Date: 2001-02-05 Completed Date: 2001-03-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804246 Medline TA: Ukr Biokhim Zh Country: Ukraine |
Other Details:
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Languages: rus Pagination: 95-104 Citation Subset: IM |
Affiliation:
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L. I. Medved Institute of Ecohygiene and Toxicology, Ukraine, Kyiv.mmarch@chdu.cv.ua |
Vernacular Title:
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Rol' ksantinoksidaznoĭ i ksantindegidrogenaznoĭ sistem v apoptoze timotsitov, indutsirovannom papaverinom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Lipid Peroxidation Papaverine / pharmacology* Rats Thymus Gland / cytology, drug effects*, metabolism Xanthine Dehydrogenase / metabolism* Xanthine Oxidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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58-74-2/Papaverine; EC 1.17.1.4/Xanthine Dehydrogenase; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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