Document Detail


Role of virus-induced myocardial affections in sudden infant death syndrome: a prospective postmortem study.
MedLine Citation:
PMID:  15155864     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cause of sudden infant death syndrome (SIDS) is an unresolved problem of high relevance. Previous studies indicate a role of infections. In our prospective study, we investigated the frequency of virus-induced myocardial affections in SIDS. Postmortem samples from SIDS victims and control subjects were investigated prospectively. Pediatric cases of unnatural death served as controls. Samples were studied for enteroviruses, adenoviruses, parvovirus B19, and Epstein-Barr virus applying PCR. Immunohistochemical investigations for inflammatory cells, the necrosis marker C5b-9((m)) complement complex, and the enteroviral capsid protein VP1 were performed. Overall, 62 SIDS victims were studied. As controls, 11 infants were enrolled. Enteroviruses were detected in 14 (22.5%), adenoviruses in 2 (3.2%), Epstein-Barr viruses in 3 (4.8%), and parvovirus B19 in 7 (11.2%) cases of SIDS. Control group samples were completely virus negative. Compared with controls, immunohistochemical investigations partially revealed a significant increase in the number of T lymphocytes in SIDS myocardial samples (p < 0.05). Furthermore, cases with elevated numbers of leukocytes and macrophages, microfocal C5b-9((m))(+) necroses, and enteroviral VP1 capsid protein within the myocardium were detected. Applying a comprehensive combination of molecular and immunohistochemical techniques, our results demonstrate a clearly higher prevalence of viral myocardial affections in SIDS. Our results emphasize the importance of PCR-based diagnosis of viral myocardial affections. We suggest preliminary criteria for cellular immunohistochemical diagnosis of viral myocardial affections derived from our findings. For future investigations in SIDS, we suggest a comprehensive approach that includes PCR and immunohistochemistry. Our results offer novel strategies for diagnosis of pediatric myocardial viral affections.
Authors:
Reinhard Dettmeyer; Anne Baasner; Marc Schlamann; Stephan A Padosch; Claudia Haag; Reinhard Kandolf; Burkhard Madea
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  55     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-24     Completed Date:  2004-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  947-52     Citation Subset:  IM    
Affiliation:
Department of Forensic Medicine, University of Bonn, Stiftsplatz 12, D-53111 Bonn, Germany. rdettmey@uni-bonn.de
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MeSH Terms
Descriptor/Qualifier:
Adenoviruses, Human / genetics,  isolation & purification
Base Sequence
Case-Control Studies
DNA, Viral / genetics
Enterovirus / genetics,  isolation & purification
Female
Herpesvirus 4, Human / genetics,  isolation & purification
Humans
Immunohistochemistry
Infant
Male
Myocarditis / complications*
Parvovirus B19, Human / genetics,  isolation & purification
Polymerase Chain Reaction
Prospective Studies
RNA, Viral / genetics,  isolation & purification
Sudden Infant Death / etiology*,  immunology,  pathology
Virus Diseases / complications*
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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