Document Detail

Role of vagal innervation on pulmonary surfactant system during fetal development.
MedLine Citation:
PMID:  19299572     Owner:  NLM     Status:  MEDLINE    
Vagally mediated afferent feedback and compliant lungs (surfactant system) play vital roles in the establishment of adequate alveolar ventilation and pulmonary gas exchange at birth. Although the significance of vagal innervation in the establishment of normal breathing patterns is well recognized, the precise role of lung innervation in the maturation of the surfactant system remains unclear. The specific aim of the present study was to investigate whether vagal denervation compromises the surfactant system during fetal development. Experiments were performed on 12 time-dated fetal sheep: 8 underwent cervical vagal denervation, and 4 were sham operated. Vagal denervation was performed at 110-113 days gestation. Fetal lambs were instrumented in utero to record arterial pH and blood-gas tensions. The animals were delivered by cesarean section under general anesthesia between 130 and 133 days gestation (term approximately 147 days). Lung samples were collected for wet-to-dry ratios, light and electron microscopy, and overall lung morphology. In addition, total proteins, total phospholipids, and surfactant proteins A and B were analyzed in both lung tissue and bronchoalveolar lavage fluid. Vagal denervation had no effect on alveolar architecture, including type II cells or the morphology of lamellar bodies within them. Furthermore, surfactant proteins A and B and total phospholipids were similar in lung tissue and bronchoalveolar lavage fluid between the two groups. A significant correlation was observed between circulating cortisol concentrations and surfactant proteins in the bronchoalveolar lavage fluid and lung tissue. We provide definitive evidence that vagal innervation at midgestation is not required for maturation of the pulmonary surfactant system during fetal development.
Luxmi Gahlot; Francis H Y Green; Anita Rigaux; Jennifer M Schneider; Shabih U Hasan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-19
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  106     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-05     Completed Date:  2009-06-11     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1641-9     Citation Subset:  IM    
Dept. of Pediatrics, Health Sciences Center, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.
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MeSH Terms
Blood Gas Analysis
Bronchoalveolar Lavage Fluid / chemistry
Fetal Development / physiology*
Gestational Age
Hydrogen-Ion Concentration
Lung / innervation*,  metabolism,  ultrastructure
Phospholipids / metabolism
Pulmonary Surfactant-Associated Protein A / analysis,  metabolism
Pulmonary Surfactant-Associated Protein B / analysis,  metabolism
Pulmonary Surfactant-Associated Proteins / analysis,  metabolism*
Pulmonary Surfactants / analysis,  metabolism*
Vagus Nerve / embryology*,  physiology*,  surgery
Reg. No./Substance:
0/Phospholipids; 0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Protein B; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants

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