Document Detail


Role of uricase in the triggering of germination of Bacillus fastidiosus spores.
MedLine Citation:
PMID:  4038258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The likelihood that uric acid was the only compound capable of triggering germination of Bacillus fastidiosus spores was reinforced by the finding that ureidoglycollic acid, urea, NH4Cl, 2,8-dihydroxypurine and a combination of L-alanine and O-carbamoyl-D-serine were ineffective as germinants. Uric acid-triggered germination of B. fastidiosus was prevented by a range of inhibitors that also inhibited uricase activity in dormant spore extracts. O2 uptake during germination started immediately after addition of uric acid, possibly as a consequence of the oxidation of uric acid by the enzyme uricase. Germination showed a dependence on uric acid concentration, with a relatively high Km (4-5 mM). During the first 10 min of germination of heat-activated spores there was no detectable change in the number of spore-cortex reducing groups, indicating that selective cortex hydrolysis is not involved in the trigger mechanism of germination of B. fastidiosus. On the basis of the results, a model is proposed in which re-initiation of uricase activity is the mechanism by which B. fastidiosus spores are triggered to emerge from the dormant state.
Authors:
J A Salas; K Johnstone; D J Ellar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  229     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-09-27     Completed Date:  1985-09-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  241-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Amidohydrolases / metabolism
Bacillus / drug effects,  enzymology,  physiology*
Calcium / metabolism
Oxygen Consumption
Picolinic Acids / metabolism
Spores, Bacterial / drug effects,  enzymology
Urate Oxidase / metabolism*
Chemical
Reg. No./Substance:
0/Picolinic Acids; 499-83-2/dipicolinic acid; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium; EC 1.7.3.3/Urate Oxidase; EC 3.5.-/Amidohydrolases; EC 3.5.2.5/allantoinase
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